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<p>Interstrand crosslinked nucleic acids offer diverse applications, including stabilizing DNA nanostructures in nanotechnology, enhancing miRNA inhibition in therapeutics, and studying enzyme activity in biochemistry. In this study, we present a new crosslinking design strategy that incorporates two 2′-deoxythioguanosine residues at noncomplementary yet strategically proximal positions within an oligo DNA duplex, promoting efficient disulfide crosslinking under chemical oxidation conditions. Additionally, this approach enables a photoinduced crosslinking reaction using 365 nm UV light, further facilitating the formation of disulfide crosslinked duplex. Our results demonstrate that the proximity of reactive thiocarbonyl groups of 2′-deoxythioguanosines incorporated within the duplex favors disulfide bond formation. Herein, we report the design strategy, crosslinking reaction results, and characterization of the crosslinked product. Furthermore, we introduce a new photoinduced disulfide crosslinking reaction and elucidate its mechanism.</p><p></p>

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Chemical and photoinduced interstrand crosslinking of oligo DNA duplexes containing 2′-deoxythioguanosines

  • 期刊论文

  • 出版模式：

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  • 发表日期：

    2025年12月23日

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  • Jamila Abbas Osman,
  • Kazumitsu Onizuka,
  • Yuuhei Yamano,
  • Fumi Nagatsugi

摘要

Interstrand crosslinked nucleic acids offer diverse applications, including stabilizing DNA nanostructures in nanotechnology, enhancing miRNA inhibition in therapeutics, and studying enzyme activity in biochemistry. In this study, we present a new crosslinking design strategy that incorporates two 2′-deoxythioguanosine residues at noncomplementary yet strategically proximal positions within an oligo DNA duplex, promoting efficient disulfide crosslinking under chemical oxidation conditions. Additionally, this approach enables a photoinduced crosslinking reaction using 365 nm UV light, further facilitating the formation of disulfide crosslinked duplex. Our results demonstrate that the proximity of reactive thiocarbonyl groups of 2′-deoxythioguanosines incorporated within the duplex favors disulfide bond formation. Herein, we report the design strategy, crosslinking reaction results, and characterization of the crosslinked product. Furthermore, we introduce a new photoinduced disulfide crosslinking reaction and elucidate its mechanism.