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<p>The detection of <i>Escherichia coli</i> (<i>E. coli</i>) in food and water is critical for public health, yet existing methods often lack sensitivity, speed, or field applicability. Here, we develop an electrochemical biosensor based on Mn-doped Co zeolitic imidazolate framework (ZIF-67) functionalized with anti-O antibody for sensitive <i>E. coli</i> detection. Mn incorporation induces phase reconstruction, surface area enhancement, and electron transfer. Antibody conjugation modulates wettability, introduces amide I and II vibrational modes, and selectively blocks electron transfer upon bacterial binding. The biosensor exhibits a linear range of 10 to 10<sup>10</sup> CFU mL<sup>–1</sup> with a 1 CFU mL<sup>–1</sup> detection limit, outperforming optical and other metal organic framework-based sensors. It can discriminate non-target bacteria (<i>Salmonella, Pseudomonas aeruginosa, Staphylococcus aureus</i>), maintain &gt;80% sensitivity over 5 weeks, and recover&#xa0;93.10 –107.52% <i>E. coli</i> spiked in tap water. This work suggests the tremendous potential for on-site pathogen monitoring.</p><p></p>

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High-performance electrochemical biosensor comprising Mn-ZIF-67 conjugated with anti-O antibody for Escherichia coli detection

  • 期刊论文

  • 出版模式：

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  • 发表日期：

    2025年10月1日

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  • Atqiya Muslihati,
  • Chandra Wulandari,
  • Ni Luh Wulan Septiani,
  • Gilang Gumilar,
  • Agus Subagio,
  • Ida Hamidah,
  • Nugraha Nugraha,
  • Erwin Peiner,
  • Hutomo Suryo Wasisto,
  • Brian Yuliarto

摘要

The detection of Escherichia coli (E. coli) in food and water is critical for public health, yet existing methods often lack sensitivity, speed, or field applicability. Here, we develop an electrochemical biosensor based on Mn-doped Co zeolitic imidazolate framework (ZIF-67) functionalized with anti-O antibody for sensitive E. coli detection. Mn incorporation induces phase reconstruction, surface area enhancement, and electron transfer. Antibody conjugation modulates wettability, introduces amide I and II vibrational modes, and selectively blocks electron transfer upon bacterial binding. The biosensor exhibits a linear range of 10 to 1010 CFU mL–1 with a 1 CFU mL–1 detection limit, outperforming optical and other metal organic framework-based sensors. It can discriminate non-target bacteria (Salmonella, Pseudomonas aeruginosa, Staphylococcus aureus), maintain >80% sensitivity over 5 weeks, and recover 93.10 –107.52% E. coli spiked in tap water. This work suggests the tremendous potential for on-site pathogen monitoring.