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<p>Parthenogenetic organisms can adapt to diverse, rapidly changing environments despite limited standing genetic variation, but the underlying molecular mechanisms remain unclear. Here, we investigate this question in the green peach aphid (<i>Myzus persicae</i>, GPA), an asexual generalist capable of colonizing a wide range of host without underlying genetic change. By integrating RNA-seq and DNA-seq from genetically identical clones reared on different hosts, we identified 1,368 high-confidence RNA editing sites (RES), many of which exhibited strong host-dependent differences in editing levels. These RES encompassed all 12 possible nucleotide substitutions, with A-to-I, C-to-U, and U-to-C conversions being particularly prevalent. Editing at several sites shifted dynamically within 48 hours of host transfer, often coinciding with changes in transcript abundance. Functional knockdown of editing genes <i>ADAR2-1</i> and <i>ADAR2-2</i> reduced editing at specific sites, diminished fecundity, and abolished survival on new hosts. Comparative analyses across aphid species reveal that although biochemical signatures of A-to-I editing are deeply conserved, the genomic locations and functional roles of edited transcripts are species-specific, reflecting lineage-specific adaptive strategies. Together, our findings highlight RNA editing as a dynamic, reversible layer of post-transcriptional regulation associated with host-dependent transcriptional plasticity in GPA, providing insights into how clonal organisms cope with environmental heterogeneity.</p>

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RNA editing fine-tunes transcriptional plasticity and enhances the adaptability of Myzus persicae to diverse host plants

  • 期刊论文

  • 出版模式：

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  • 发表日期：

    2026年7月23日

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  • Wenyuan Yu,
  • Jun Wu,
  • Rong Hu,
  • Zhimou Lin,
  • Peiyu Yang,
  • Zhichao Hu,
  • Pengshuai Peng,
  • Shuai Zhan,
  • Jean-Christophe Simon,
  • Saskia A. Hogenhout,
  • Gang Wu,
  • Shuangxia Jin,
  • Yazhou Chen

摘要

Parthenogenetic organisms can adapt to diverse, rapidly changing environments despite limited standing genetic variation, but the underlying molecular mechanisms remain unclear. Here, we investigate this question in the green peach aphid (Myzus persicae, GPA), an asexual generalist capable of colonizing a wide range of host without underlying genetic change. By integrating RNA-seq and DNA-seq from genetically identical clones reared on different hosts, we identified 1,368 high-confidence RNA editing sites (RES), many of which exhibited strong host-dependent differences in editing levels. These RES encompassed all 12 possible nucleotide substitutions, with A-to-I, C-to-U, and U-to-C conversions being particularly prevalent. Editing at several sites shifted dynamically within 48 hours of host transfer, often coinciding with changes in transcript abundance. Functional knockdown of editing genes ADAR2-1 and ADAR2-2 reduced editing at specific sites, diminished fecundity, and abolished survival on new hosts. Comparative analyses across aphid species reveal that although biochemical signatures of A-to-I editing are deeply conserved, the genomic locations and functional roles of edited transcripts are species-specific, reflecting lineage-specific adaptive strategies. Together, our findings highlight RNA editing as a dynamic, reversible layer of post-transcriptional regulation associated with host-dependent transcriptional plasticity in GPA, providing insights into how clonal organisms cope with environmental heterogeneity.