Scholarlink

<p>Insect-specific juvenile hormone (JH) controls mosquito post-eclosion (PE) development, a stage crucial for subsequent blood meal-driven egg maturation. During PE, JH functions through the independent or cumulative actions of repressors Hairy and Krüppel homolog 1 (Kr-h1), initiating the downregulation of different target gene cohorts. A key factor governing candidate gene expression is its chromatin state, determined by the tightness of DNA wrapping around associated proteins. Chromatin remodeling alters DNA accessibility and impacts gene expression. Despite advances in our understanding of the JH signaling pathway, little is known about JH/Met-induced chromatin remodeling during gene repression. Using the Dengue mosquito <i>Aedes aegypti</i>, we show that repressors Hairy and Kr-h1 induce alternative chromatin modifications to repress their target genes. While Hairy causes widespread histone H4 deacetylation of target genes, Kr-h1 induces histone H3K9 trimethylation and chromatin compaction. For the candidate genes co-repressed by both Hairy and Kr-h1, the two factors have a cumulative effect on the chromatin modification of the target gene loci, downregulating their expression. Our findings shed light on the mechanism behind the cumulative action of transcriptional repressors Hairy and Kr-h1 involved in JH signal transduction.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Juvenile hormone-mediated chromatin remodelling by cumulative action of downstream transcription factors, Hairy and Krüppel homolog 1

  • 期刊论文

  • 出版模式：

    开放获取

  • 发表日期：

    2026年5月26日

查看全文
  • Roumik Banerjee,
  • Challiyil S. Jayapriya,
  • Ajeet K. Mohanty,
  • Alexander S. Raikhel,
  • Tusar T. Saha

摘要

Insect-specific juvenile hormone (JH) controls mosquito post-eclosion (PE) development, a stage crucial for subsequent blood meal-driven egg maturation. During PE, JH functions through the independent or cumulative actions of repressors Hairy and Krüppel homolog 1 (Kr-h1), initiating the downregulation of different target gene cohorts. A key factor governing candidate gene expression is its chromatin state, determined by the tightness of DNA wrapping around associated proteins. Chromatin remodeling alters DNA accessibility and impacts gene expression. Despite advances in our understanding of the JH signaling pathway, little is known about JH/Met-induced chromatin remodeling during gene repression. Using the Dengue mosquito Aedes aegypti, we show that repressors Hairy and Kr-h1 induce alternative chromatin modifications to repress their target genes. While Hairy causes widespread histone H4 deacetylation of target genes, Kr-h1 induces histone H3K9 trimethylation and chromatin compaction. For the candidate genes co-repressed by both Hairy and Kr-h1, the two factors have a cumulative effect on the chromatin modification of the target gene loci, downregulating their expression. Our findings shed light on the mechanism behind the cumulative action of transcriptional repressors Hairy and Kr-h1 involved in JH signal transduction.