Scholarlink

<p>Wheat powdery mildew, caused by <i>Blumeria graminis</i> f. sp. <i>tritici</i> (<i>Bgt</i>), severely threatens global wheat production. In this study, the cultivated emmer accession CWI16926-4Y exhibits high-level and broad-spectrum resistance at both seedling and adult plant stages. Genetic analysis reveals that the powdery mildew resistance in CWI16926-4Y is controlled by a single dominant gene, designated <i>PmCWI16926</i>. Employing bulked segregant RNA analysis combined with high-density mapping, a 590 kb physical interval (21.70-22.29 Mb) on chromosome 2BS is delimited and referenced against the durum wheat cv. Svevo genome assembly. Within the interval, we identify two NLR-type genes, with <i>TRITD2Bv1G010140</i> emerging as the most promising candidate based on pathogen-induced expression and a unique haplotype. Moreover, we validate four co-segregated markers and a gene-specific marker, enabling marker-assisted transfer of <i>PmCWI16926</i> into elite cultivars in breeding. This study expands the repertoire of deployable <i>Pm</i> genes from emmer wheat and provides additional genetic tools for resistance breeding.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Fine mapping of a powdery mildew resistance gene PmCWI16926 from cultivated emmer wheat

  • 期刊论文

  • 出版模式：

    开放获取

  • 发表日期：

    2026年4月1日

查看全文
  • Xiaozhe Xu,
  • Dongming Li,
  • Fengtao Wang,
  • Yuli Jin,
  • Kai Wang,
  • Nina Sun,
  • Linzhi Li,
  • Jiatong Li,
  • Yintao Dai,
  • Tao Zhang,
  • Cheng Liu,
  • Guohao Han,
  • Ningning Yu

摘要

Wheat powdery mildew, caused by Blumeria graminis f. sp. tritici (Bgt), severely threatens global wheat production. In this study, the cultivated emmer accession CWI16926-4Y exhibits high-level and broad-spectrum resistance at both seedling and adult plant stages. Genetic analysis reveals that the powdery mildew resistance in CWI16926-4Y is controlled by a single dominant gene, designated PmCWI16926. Employing bulked segregant RNA analysis combined with high-density mapping, a 590 kb physical interval (21.70-22.29 Mb) on chromosome 2BS is delimited and referenced against the durum wheat cv. Svevo genome assembly. Within the interval, we identify two NLR-type genes, with TRITD2Bv1G010140 emerging as the most promising candidate based on pathogen-induced expression and a unique haplotype. Moreover, we validate four co-segregated markers and a gene-specific marker, enabling marker-assisted transfer of PmCWI16926 into elite cultivars in breeding. This study expands the repertoire of deployable Pm genes from emmer wheat and provides additional genetic tools for resistance breeding.