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<p>Understanding how neural progenitors generate diverse neuronal subtypes is central to developmental and evolutionary neuroscience. Lineage tracing is a key tool for dissecting these processes, but existing methods are often constrained by stochastic expression, recombination bias, or reliance on transgenic models—limitations that restrict their application in non-model organisms. Here, we present an enhanced iOn switch system optimized for evo-devo research. Leveraging its modular, integration-based transgene expression logic, we developed a tunable labeling strategy that enables both sparse and dense labeling within a single framework. We further expanded the system’s fluorescent palette and introduced subcellular targeting options to support multiclonal analysis and stable time-lapse imaging. Finally, we demonstrate the versatility of this approach across a wide range of vertebrate species, including mouse, guinea pig, rat, chick, turtle, and zebrafish, thereby establishing the iOn switch system as a flexible and accessible tool for evo-devo research.</p>

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Expanded iOn switch toolkit enables flexible clonal labeling and dynamic imaging in model and non-model animals

  • 期刊论文

  • 出版模式：

    开放获取

  • 发表日期：

    2026年3月24日

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  • Zi Chao Ngiam,
  • Kyosuke Wada,
  • Jun Hatakeyama,
  • Yuki Y. Yamauchi,
  • Takuya Kaneko,
  • Pauline Rouillard,
  • Haruka Sato,
  • Masahiko Hibi,
  • Ikuo K. Suzuki,
  • Carina Hanashima,
  • Chiaki Ohtaka-Maruyama,
  • Takuma Kumamoto

摘要

Understanding how neural progenitors generate diverse neuronal subtypes is central to developmental and evolutionary neuroscience. Lineage tracing is a key tool for dissecting these processes, but existing methods are often constrained by stochastic expression, recombination bias, or reliance on transgenic models—limitations that restrict their application in non-model organisms. Here, we present an enhanced iOn switch system optimized for evo-devo research. Leveraging its modular, integration-based transgene expression logic, we developed a tunable labeling strategy that enables both sparse and dense labeling within a single framework. We further expanded the system’s fluorescent palette and introduced subcellular targeting options to support multiclonal analysis and stable time-lapse imaging. Finally, we demonstrate the versatility of this approach across a wide range of vertebrate species, including mouse, guinea pig, rat, chick, turtle, and zebrafish, thereby establishing the iOn switch system as a flexible and accessible tool for evo-devo research.