Scholarlink

<p><i>Theileria annulata</i>-infected host leukocytes exhibit cancer-like phenotypes, driven by mechanisms that remain incompletely understood. This study explores the genomic alterations underlying these transformations using whole-genome sequencing and bioinformatic analyses of six clinically relevant <i>T. annulata</i>-infected cell lines. Here we identify 7867 exon-linked somatic mutations shared across all cell lines, with significant enrichment in oncogenes (e.g., <i>FLT4, NOTCH2, MAP3K1, DAXX, FCGR2B, ROS1</i>) and tumor suppressor genes (e.g., <i>BARD1, KMT2C, GRIN2A, BAP1</i>). These mutations are associated with critical cancer-related pathways. Functional studies revealed that inhibition of the mutated oncogene <i>ROS1</i> using crizotinib induces death in infected leukocytes, confirming its role in transformation. Additionally, we observe mutations in genes linked to genomic instability and the DNA damage response (DDR) pathways, highlighting potential parallels with cancer biology. Suppression of TP53, a key tumor suppressor, is implicated in the immortalization of infected cells, while upregulation of the DNA mutator enzyme APOBEC3H suggests a parasite-driven, mutation-inducing mechanism. Our findings provide new insights into how <i>T. annulata</i> reprograms host cells through genomic instability and mutations, identifying <i>ROS1</i> and <i>TP53</i> as critical targets for therapeutic intervention. This work advances understanding of parasite-induced oncogenic transformation and offers pathways for future research.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Genomic characterization of host gene alterations in Theileria annulata-transformed leukocytes

  • 期刊论文

  • 出版模式：

    开放获取

  • 发表日期：

    2025年11月20日

查看全文
  • Debabrata Dandasena,
  • Akash Suresh,
  • Roli Budhwar,
  • Jeffrey Godwin,
  • Sakshi Singh,
  • Madhusmita Subudhi,
  • Vengatachala Moorthy A,
  • Amruthanjali T,
  • Sonti Roy,
  • Vasundhra Bhandari,
  • Paresh Sharma

摘要

Theileria annulata-infected host leukocytes exhibit cancer-like phenotypes, driven by mechanisms that remain incompletely understood. This study explores the genomic alterations underlying these transformations using whole-genome sequencing and bioinformatic analyses of six clinically relevant T. annulata-infected cell lines. Here we identify 7867 exon-linked somatic mutations shared across all cell lines, with significant enrichment in oncogenes (e.g., FLT4, NOTCH2, MAP3K1, DAXX, FCGR2B, ROS1) and tumor suppressor genes (e.g., BARD1, KMT2C, GRIN2A, BAP1). These mutations are associated with critical cancer-related pathways. Functional studies revealed that inhibition of the mutated oncogene ROS1 using crizotinib induces death in infected leukocytes, confirming its role in transformation. Additionally, we observe mutations in genes linked to genomic instability and the DNA damage response (DDR) pathways, highlighting potential parallels with cancer biology. Suppression of TP53, a key tumor suppressor, is implicated in the immortalization of infected cells, while upregulation of the DNA mutator enzyme APOBEC3H suggests a parasite-driven, mutation-inducing mechanism. Our findings provide new insights into how T. annulata reprograms host cells through genomic instability and mutations, identifying ROS1 and TP53 as critical targets for therapeutic intervention. This work advances understanding of parasite-induced oncogenic transformation and offers pathways for future research.