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<p>The entomophilic roundworm <i>Pristionchus pacificus</i> produces a group of complex ascaroside pheromones (e.g., ubas#1), which are built with various intermediates originating from primary metabolic pathways. However, the exact biosynthetic pathways resulting in the production of these modular pheromones remain enigmatic. Here, the application of a workflow combing bioinformatic tools, CRISPR engineering and chemical analysis supports the discovery of three new carboxylesterases <i>Ppa</i>-UAR-5, <i>Ppa</i>-UAR-6 and <i>Ppa</i>-UAR-12 from <i>P. pacificus</i>. <i>Ppa</i>-UAR-5 links two simple ascarosides oscr#9 and ascr#12 to the 2’-position of ubas#3 to furnish the biosynthesis of ubas#1 and ubas#2, whereas <i>Ppa</i>-UAR-12 links two ascr#1 at the 4’-position to synthesize dasc#1. Finally, <i>Ppa</i>-UAR-6 is essential for the biosynthesis of npar#1-3 and part#9. The expression patterns of <i>Ppa-uar-6</i> and <i>Ppa-uar-12</i> in intestinal and epidermal cells further suggest pheromone biosynthesis is tissue-specific. These findings support the notion that the expansion and subsequent diversification of carboxylesterases in <i>P. pacificus</i> generates complex modular signaling pheromones.</p><p></p>

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Biosynthesis of modular signaling molecules requires functional diversification of carboxylesterases in Pristionchus pacificus

  • 期刊论文

  • 出版模式：

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  • 发表日期：

    2025年8月13日

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  • Pei Zhang,
  • Penghieng Theam,
  • Wenya Xu,
  • Shudan Ye,
  • Hanh Witte,
  • Tingwei Liu,
  • Ralf J. Sommer,
  • Chuanfu Dong

摘要

The entomophilic roundworm Pristionchus pacificus produces a group of complex ascaroside pheromones (e.g., ubas#1), which are built with various intermediates originating from primary metabolic pathways. However, the exact biosynthetic pathways resulting in the production of these modular pheromones remain enigmatic. Here, the application of a workflow combing bioinformatic tools, CRISPR engineering and chemical analysis supports the discovery of three new carboxylesterases Ppa-UAR-5, Ppa-UAR-6 and Ppa-UAR-12 from P. pacificus. Ppa-UAR-5 links two simple ascarosides oscr#9 and ascr#12 to the 2’-position of ubas#3 to furnish the biosynthesis of ubas#1 and ubas#2, whereas Ppa-UAR-12 links two ascr#1 at the 4’-position to synthesize dasc#1. Finally, Ppa-UAR-6 is essential for the biosynthesis of npar#1-3 and part#9. The expression patterns of Ppa-uar-6 and Ppa-uar-12 in intestinal and epidermal cells further suggest pheromone biosynthesis is tissue-specific. These findings support the notion that the expansion and subsequent diversification of carboxylesterases in P. pacificus generates complex modular signaling pheromones.