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<p>Fibroblasts are abundant throughout the body and easily accessible without animal sacrifice. They possess the ability to proliferate and differentiate into muscle and fat tissues, as well as to produce extracellular matrix components, making them ideal for cultured meat production. In this study, we utilized the pig fibroblasts as seed cells and conducted myogenic/lipogenic transdifferentiation in 3D to create a muscle/fat matrix constituting whole-cut meat within 9 days. The muscle cells were efficiently derived from the fibroblasts overexpressing <i>MyoD</i> and the subsequent lipid deposition into the muscle was achieved by supplementing with a lipogenic inducer composed of olive oil and soybean lecithin. Lipidomic analysis revealed that the engineered meat exhibited a healthier fatty acid profile compare to conventional pork, with reduced saturated fatty acids (44.49% vs. 51.2%) and increased polyunsaturated fatty acids (31.33% vs. 27.01%). These findings open new avenues for customized cultured meat production with optimized fatty acids compositions tailored to meet the diverse consumer demands.</p>

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Optimizing fatty acids composition in meat-like tissue derived from myogenic conversion of pig fibroblasts

  • 期刊论文

  • 出版模式：

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  • 发表日期：

    2025年7月29日

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  • Pengxiang Zhao,
  • Jianqi Lv,
  • Wei Gu,
  • Hao Mi,
  • Haiying Cui,
  • Lei Chen,
  • Alexander Kurdeko,
  • Heng Wang

摘要

Fibroblasts are abundant throughout the body and easily accessible without animal sacrifice. They possess the ability to proliferate and differentiate into muscle and fat tissues, as well as to produce extracellular matrix components, making them ideal for cultured meat production. In this study, we utilized the pig fibroblasts as seed cells and conducted myogenic/lipogenic transdifferentiation in 3D to create a muscle/fat matrix constituting whole-cut meat within 9 days. The muscle cells were efficiently derived from the fibroblasts overexpressing MyoD and the subsequent lipid deposition into the muscle was achieved by supplementing with a lipogenic inducer composed of olive oil and soybean lecithin. Lipidomic analysis revealed that the engineered meat exhibited a healthier fatty acid profile compare to conventional pork, with reduced saturated fatty acids (44.49% vs. 51.2%) and increased polyunsaturated fatty acids (31.33% vs. 27.01%). These findings open new avenues for customized cultured meat production with optimized fatty acids compositions tailored to meet the diverse consumer demands.