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<p>The WZ sex determination system is found in a diverse range of animals, including lepidopteran insects. In the silkworm <i>Bombyx mori</i>, the masculinizing protein Masculinizer (BmMasc) induces the production of the male-type <i>Bombyx mori doublesex</i> (<i>Bmdsx</i><sup><i>M</i></sup>), which is the master genetic switch of <i>B. mori</i> sex differentiation. However, the molecular mechanism through which BmMasc transduces the masculinizing signal to the <i>Bmdsx</i><sup><i>M</i></sup> production remains unknown. Here, we show that BmMasc physically interacts with <i>Bombyx mori</i> P-element somatic inhibitor (BmPSI), a RNA binding protein required for <i>Bmdsx</i><sup><i>M</i></sup> expression. Knockdown experiments indicate that BmPSI is essential for the masculinizing activity of BmMasc. RNA immunoprecipitation experiments also reveal that BmMasc-containing protein complex is associated with female-specific regions of <i>Bmdsx</i> pre-mRNA. Taken together, our findings show that the BmMasc–BmPSI protein complex binds to female-specific <i>Bmdsx</i> regions, inducing exon skipping, and thereby promoting <i>Bmdsx</i><sup><i>M</i></sup> expression in <i>B. mori</i> males.</p>

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The Masc–PSI complex directly induces male-type doublesex splicing in silkworms

  • 期刊论文

  • 出版模式：

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  • 发表日期：

    2025年6月14日

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  • Tatsunori Kaneda,
  • Noriko Matsuda-Imai,
  • Hidetaka Kosako,
  • Keisuke Shoji,
  • Masataka G. Suzuki,
  • Yutaka Suzuki,
  • Takashi Kiuchi,
  • Susumu Katsuma

摘要

The WZ sex determination system is found in a diverse range of animals, including lepidopteran insects. In the silkworm Bombyx mori, the masculinizing protein Masculinizer (BmMasc) induces the production of the male-type Bombyx mori doublesex (BmdsxM), which is the master genetic switch of B. mori sex differentiation. However, the molecular mechanism through which BmMasc transduces the masculinizing signal to the BmdsxM production remains unknown. Here, we show that BmMasc physically interacts with Bombyx mori P-element somatic inhibitor (BmPSI), a RNA binding protein required for BmdsxM expression. Knockdown experiments indicate that BmPSI is essential for the masculinizing activity of BmMasc. RNA immunoprecipitation experiments also reveal that BmMasc-containing protein complex is associated with female-specific regions of Bmdsx pre-mRNA. Taken together, our findings show that the BmMasc–BmPSI protein complex binds to female-specific Bmdsx regions, inducing exon skipping, and thereby promoting BmdsxM expression in B. mori males.