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<p>Immunoglobulin A nephropathy (IgA-N) is a primary glomerulonephritis that is characterized by mesangial cell proliferation and expansion. Although glomerular endothelial cells (GE) are implicated in the pathogenesis of IgA-N, their role remains poorly understood. We conduct single-cell and spatial transcriptomic analysis using human specimens with mild IgA-N compared with normal. We integrate single-cell and spatial transcriptome analyses in human samples of mild IgA-N compared to normal samples, revealing several novel findings: (1) identification of clusters of GE and their expressed gene profiles, (2) identification of novel inflammation-related molecules newly implicated in GE in mild IgA-N, (3) activation of inflammatory pathways characteristic of IgA-N. Therefore, we suggest the importance of GE in the mechanism of IgA-N, as GE initiates more active inflammatory response prior to mesangial cells in patients with mild IgA-N. These findings provide useful information for understanding the pathogenesis and choosing the best drug for IgA nephropathy.</p><p></p>

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Inflammation-associated molecules in the glomerular-endothelium in mild IgA-nephropathy patients identified by single-cell and spatial transcriptome

  • 期刊论文

  • 出版模式：

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  • 发表日期：

    2025年6月12日

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  • Kumi Hasegawa,
  • Nagako Kawashima,
  • Ayako Kawabata,
  • Megumi Sakakura,
  • Naoki Onoda,
  • Takashi Sano,
  • Itaru Urakawa,
  • Masahiro Matsubara,
  • Shokichi Naito

摘要

Immunoglobulin A nephropathy (IgA-N) is a primary glomerulonephritis that is characterized by mesangial cell proliferation and expansion. Although glomerular endothelial cells (GE) are implicated in the pathogenesis of IgA-N, their role remains poorly understood. We conduct single-cell and spatial transcriptomic analysis using human specimens with mild IgA-N compared with normal. We integrate single-cell and spatial transcriptome analyses in human samples of mild IgA-N compared to normal samples, revealing several novel findings: (1) identification of clusters of GE and their expressed gene profiles, (2) identification of novel inflammation-related molecules newly implicated in GE in mild IgA-N, (3) activation of inflammatory pathways characteristic of IgA-N. Therefore, we suggest the importance of GE in the mechanism of IgA-N, as GE initiates more active inflammatory response prior to mesangial cells in patients with mild IgA-N. These findings provide useful information for understanding the pathogenesis and choosing the best drug for IgA nephropathy.