Scholarlink

<p>Biological methods are a promising route for the environmentally-friendly production of rare earth elements (REE), which are essential for sustainable energy and defense technologies. In earlier work we identified the key genetic mechanisms contributing to the REE-bioleaching capability of <i>Gluconobacter oxydans</i> B58. Here we have targeted two of these mechanisms to generate a high-efficiency bioleaching strain of <i>G. oxydans</i>. Disruption of the phosphate-specific transport system through a clean deletion of <i>pstS</i> constitutively turns on the phosphate starvation response, yielding a much more acidic biolixiviant, and increasing bioleaching by up to 30%. Coupling knockout of <i>pstS</i> with the over-expression of the <i>mgdh</i> membrane-bound glucose dehydrogenase gene using the P<sub>112</sub> promoter (strain <i>G. oxydans</i> Δ<i>pstS</i>, P<sub>112</sub>:<i>mgdh</i>) reduces biolixiviant pH by 0.39 units; increases REE-bioleaching by 53% at a pulp density of 10% and increases it by 73% at a pulp density of 1%.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

High efficiency rare earth element bioleaching with systems biology guided engineering of Gluconobacter oxydans

  • 期刊论文

  • 出版模式：

    开放获取

  • 发表日期：

    2025年5月27日

查看全文
  • Alexa M. Schmitz,
  • Brooke Pian,
  • Sabrina Marecos,
  • Mingming Wu,
  • Megan Holycross,
  • Esteban Gazel,
  • Matthew C. Reid,
  • Buz Barstow

摘要

Biological methods are a promising route for the environmentally-friendly production of rare earth elements (REE), which are essential for sustainable energy and defense technologies. In earlier work we identified the key genetic mechanisms contributing to the REE-bioleaching capability of Gluconobacter oxydans B58. Here we have targeted two of these mechanisms to generate a high-efficiency bioleaching strain of G. oxydans. Disruption of the phosphate-specific transport system through a clean deletion of pstS constitutively turns on the phosphate starvation response, yielding a much more acidic biolixiviant, and increasing bioleaching by up to 30%. Coupling knockout of pstS with the over-expression of the mgdh membrane-bound glucose dehydrogenase gene using the P112 promoter (strain G. oxydans ΔpstS, P112:mgdh) reduces biolixiviant pH by 0.39 units; increases REE-bioleaching by 53% at a pulp density of 10% and increases it by 73% at a pulp density of 1%.