Cross-niche protection of kiwi plant against above-ground canker disease by beneficial rhizosphere Flavobacterium

Beneficial rhizosphere microorganisms are widely employed to shield crops from underground pathogen infections. In this study, we challenge this conventional idea by employing rhizosphere soil bacteria to safeguard kiwi plants against the above-ground canker, caused by Pseudomonas syringae pv. actinidiae (Psa). Microbiome comparisons were conducted in different resistant cultivars Actinidia chinensis var. deliciosa ‘Hayward’ and A. chinensis var. chinensis ‘Hongyang’. Our findings reveal the most notable disparity in the rhizosphere soil microbiome, with the Flavobacterium significantly enriched in the rhizosphere soil of more resistant cultivar, ‘Hayward’. We isolated Flavobacterium isolates and observed their efficacy in preventing Psa infection, which is further confirmed in field trial by using a representative strain Flavobacterium soyae F55. Furthermore, undescribed gene clusters responsible for antimicrobial metabolite biosynthesis were identified in F. soyae F55, and F. soyae F55 growth was evidently promoted by the root exudates of ‘Hayward’. The results underscore the potential of beneficial rhizosphere soil bacteria in protection against above-ground disease.