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<p>Microbe–semiconductor hybrids hold promise for solar-to-chemical conversion, but facet-dependent interfacial charge transfer remains poorly understood due to structural heterogeneity and biological complexity. Here we leverage a multimodal optical imaging platform to probe the charge-transfer efficiency between <i>Shewanella oneidensis</i> MR-1 and {110}/{001}-faceted haematite, at single-particle and single-cell levels, in vivo and operando. We quantify the reverse extracellular electron-transfer capabilities of <i>Shewanella oneidensis</i> MR-1 via non-H<sub>2</sub>-mediated pathways, and identify that haematite’s {110} facets synergistically exhibit stronger cell-binding ability and higher charge-transfer efficiency. Furthermore, we discover that moderate cell densities are key to enhancing per-cell electron injection, highlighting the trade-off between total loading and individual cell efficiency, and offering critical insights into biofilm structure optimization. Our imaging tools and analytical framework may potentially extend to diverse microbe–semiconductor hybrid systems, quantifying microscopic structural and functional descriptors that enhance the fundamental understanding of complex interfacial charge transfer, and inform rational biohybrid design across applications.</p><p></p>

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Single-particle imaging uncovers reverse electron transfer efficiency between Shewanella oneidensis MR-1 and shaped haematite

  • 期刊论文

  • 发表日期：

    2026年4月28日

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  • Yong Liu,
  • Wentao Song,
  • Weidong Zhang,
  • Yuanmei Liang,
  • Mukesh Saini,
  • Yuanzhi He,
  • Jing Zhu,
  • Zhongxin Chen,
  • Guoliang Zhang,
  • Jin Xie,
  • Xiaozhi Xu,
  • Guillermo C. Bazan,
  • Jee Loon Foo,
  • Matthew Wook Chang,
  • Bin Liu,
  • Xianwen Mao

摘要

Microbe–semiconductor hybrids hold promise for solar-to-chemical conversion, but facet-dependent interfacial charge transfer remains poorly understood due to structural heterogeneity and biological complexity. Here we leverage a multimodal optical imaging platform to probe the charge-transfer efficiency between Shewanella oneidensis MR-1 and {110}/{001}-faceted haematite, at single-particle and single-cell levels, in vivo and operando. We quantify the reverse extracellular electron-transfer capabilities of Shewanella oneidensis MR-1 via non-H2-mediated pathways, and identify that haematite’s {110} facets synergistically exhibit stronger cell-binding ability and higher charge-transfer efficiency. Furthermore, we discover that moderate cell densities are key to enhancing per-cell electron injection, highlighting the trade-off between total loading and individual cell efficiency, and offering critical insights into biofilm structure optimization. Our imaging tools and analytical framework may potentially extend to diverse microbe–semiconductor hybrid systems, quantifying microscopic structural and functional descriptors that enhance the fundamental understanding of complex interfacial charge transfer, and inform rational biohybrid design across applications.