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<p>Biological wastewater treatment systems with low carbon footprints and capability of resource recovery are pivotal to sustainable wastewater management. Identifying microbial agents for sustainable phosphorus recovery is indispensable yet challenging because of the knowledge gap in phylogenic diversity and the insufficiency of current identification methods. Here, leveraging phenotype-targeted metagenomics coupled with label-free Raman-activated single-cell sorting, we discover <i>Candidatus Methylobacter multiphosphori</i> as a methanotrophic polyphosphate-accumulating organism (PAO) for methane-driven, low-carbon phosphorus recovery. Comparative genomic analysis and computational evolutionary analyses reveal their dual roles as a methane sink and a polyphosphate accumulator, implying their unexplored yet probably broad involvement in both carbon and phosphorus cycling across various environments. Mixed methanotrophic PAOs enriched from an actual digestate-fed methane bioreactor verify that methane is used to drive polyphosphate accumulations, reducing carbon footprints of phosphorus recovery. Lastly, techno-economic analyses quantify the potential of methanotrophic PAOs in reducing greenhouse gas emissions and operation costs of manure wastewater treatment in the USA. Overall, this study offers opportunities to propel circular phosphorus economy and sustainable wastewater management.</p>

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Methanotroph-driven phosphorus recovery for sustainable wastewater management

  • 期刊论文

  • 发表日期：

    2026年5月11日

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  • Zijian Wang,
  • Hong-Zhe Li,
  • Yanfei Tang,
  • Guangyu Li,
  • Dongqi Wang,
  • Yuan Yan,
  • Peisheng He,
  • Hua Cai,
  • Yong-Guan Zhu,
  • Li Cui,
  • April Z. Gu

摘要

Biological wastewater treatment systems with low carbon footprints and capability of resource recovery are pivotal to sustainable wastewater management. Identifying microbial agents for sustainable phosphorus recovery is indispensable yet challenging because of the knowledge gap in phylogenic diversity and the insufficiency of current identification methods. Here, leveraging phenotype-targeted metagenomics coupled with label-free Raman-activated single-cell sorting, we discover Candidatus Methylobacter multiphosphori as a methanotrophic polyphosphate-accumulating organism (PAO) for methane-driven, low-carbon phosphorus recovery. Comparative genomic analysis and computational evolutionary analyses reveal their dual roles as a methane sink and a polyphosphate accumulator, implying their unexplored yet probably broad involvement in both carbon and phosphorus cycling across various environments. Mixed methanotrophic PAOs enriched from an actual digestate-fed methane bioreactor verify that methane is used to drive polyphosphate accumulations, reducing carbon footprints of phosphorus recovery. Lastly, techno-economic analyses quantify the potential of methanotrophic PAOs in reducing greenhouse gas emissions and operation costs of manure wastewater treatment in the USA. Overall, this study offers opportunities to propel circular phosphorus economy and sustainable wastewater management.