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<p>A 63-year-old female who presented with bilateral panuveitis suspicious for a neoplastic etiology, underwent diagnostic pars plana vitrectomy of her right eye. Cytology was negative and flow cytometry did not reveal evidence of B-cell lymphoma. PCR revealed T-cell Receptor Gamma (TRG) clonal peaks and NGS demonstrated mutations in the <i>DNMT3A</i> and <i>MAP2K1</i> genes. Systemic oncologic surveillance was inconclusive, but flow cytometry of peripheral blood revealed an expansion of aberrant T-cells. Subsequent vitreous biopsy of the fellow eye revealed identical TRG clonal peaks, and T-cell flow cytometry immunophenotype results were similar to those in peripheral blood, with a TRBC1 monotypic pattern, consistent with a diagnosis of bilateral T-cell vitreoretinal lymphoma. T-cell vitreoretinal lymphomas present a diagnostic challenge, which can often delay treatment. This case highlights the usefulness of molecular techniques, such as PCR, flow cytometry and NGS, to complement conventional diagnostic techniques.</p>

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Molecular analysis of bilateral T-cell vitreoretinal lymphoma

  • 期刊论文

  • 出版模式：

    开放获取

  • 发表日期：

    2025年7月18日

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  • Matias Soifer,
  • Sonny Caplash,
  • Rajesh C. Rao,
  • Hao-Wei Wang,
  • Constance M. Yuan,
  • Antonios Papanicolau-Sengos,
  • Liqiang Xi,
  • Sunil Bellur,
  • Shilpa Kodati

摘要

A 63-year-old female who presented with bilateral panuveitis suspicious for a neoplastic etiology, underwent diagnostic pars plana vitrectomy of her right eye. Cytology was negative and flow cytometry did not reveal evidence of B-cell lymphoma. PCR revealed T-cell Receptor Gamma (TRG) clonal peaks and NGS demonstrated mutations in the DNMT3A and MAP2K1 genes. Systemic oncologic surveillance was inconclusive, but flow cytometry of peripheral blood revealed an expansion of aberrant T-cells. Subsequent vitreous biopsy of the fellow eye revealed identical TRG clonal peaks, and T-cell flow cytometry immunophenotype results were similar to those in peripheral blood, with a TRBC1 monotypic pattern, consistent with a diagnosis of bilateral T-cell vitreoretinal lymphoma. T-cell vitreoretinal lymphomas present a diagnostic challenge, which can often delay treatment. This case highlights the usefulness of molecular techniques, such as PCR, flow cytometry and NGS, to complement conventional diagnostic techniques.