Scholarlink

<p>Tomato leaf curl Palampur virus (ToLCPalV; <i>Begomovirus solanumpalampurense</i>) is an emerging threat to tomato production in Southwest Asia, with limited effective management options. This study investigates the potential of seed priming using <i>Trichoderma zelobreve</i>, <i>Bacillus subtilis</i>, and the chemical elicitor β-aminobutyric acid (BABA), individually and in combination, to mitigate ToLCPalV infection in two tomato cultivars, Moneymaker and Tabas. Seedlings were agroinoculated with an infectious clone of ToLCPalV and assessed 40 days post-inoculation for symptom severity, viral DNA accumulation, plant growth parameters, antioxidant enzyme activities, and defense gene expression. Results revealed that primed plants exhibited significantly reduced symptom severity and viral accumulation when treated with the triple combination of BABA, <i>B</i>. <i>subtilis</i>, and <i>T</i>. <i>zelobreve</i> (BaBT). Moneymaker displayed stronger priming responses than Tabas, correlating with enhanced expression of the jasmonic acid pathway gene <i>SlPI-II</i> and elevated activities of key antioxidant enzymes (polyphenol oxidase, peroxidase, catalase). Expression of the ethylene-related gene <i>SlPTI4</i> was associated with increased susceptibility. Root colonization tests confirmed the persistence of biocontrol agents in the rhizosphere, and ToLCPa1V was not detected in seeds, F1, or Fr progeny through PCR analysis. These findings show that microbial and chemical seed priming effectively induces systemic antiviral protection, suppresses ToLCPalV infection, and promotes healthier plant growth under the ToLCPalV challenge. This integrated approach is a promising and sustainable strategy for managing ToLCPalV and potentially other viral diseases in tomato production.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Microbial and β-aminobutyric acid priming mitigate tomato leaf curl Palampur virus infection in tomato plants through different mechanisms

  • 期刊论文

  • 出版模式：

    开放获取

  • 发表日期：

    2026年8月4日

查看全文
  • Roya Kazemi,
  • Naser Safaie,
  • Mohammad Reza Atighi,
  • Masoud Shams-Bakhsh

摘要

Tomato leaf curl Palampur virus (ToLCPalV; Begomovirus solanumpalampurense) is an emerging threat to tomato production in Southwest Asia, with limited effective management options. This study investigates the potential of seed priming using Trichoderma zelobreve, Bacillus subtilis, and the chemical elicitor β-aminobutyric acid (BABA), individually and in combination, to mitigate ToLCPalV infection in two tomato cultivars, Moneymaker and Tabas. Seedlings were agroinoculated with an infectious clone of ToLCPalV and assessed 40 days post-inoculation for symptom severity, viral DNA accumulation, plant growth parameters, antioxidant enzyme activities, and defense gene expression. Results revealed that primed plants exhibited significantly reduced symptom severity and viral accumulation when treated with the triple combination of BABA, B. subtilis, and T. zelobreve (BaBT). Moneymaker displayed stronger priming responses than Tabas, correlating with enhanced expression of the jasmonic acid pathway gene SlPI-II and elevated activities of key antioxidant enzymes (polyphenol oxidase, peroxidase, catalase). Expression of the ethylene-related gene SlPTI4 was associated with increased susceptibility. Root colonization tests confirmed the persistence of biocontrol agents in the rhizosphere, and ToLCPa1V was not detected in seeds, F1, or Fr progeny through PCR analysis. These findings show that microbial and chemical seed priming effectively induces systemic antiviral protection, suppresses ToLCPalV infection, and promotes healthier plant growth under the ToLCPalV challenge. This integrated approach is a promising and sustainable strategy for managing ToLCPalV and potentially other viral diseases in tomato production.