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<p>Blue light exposure significantly increases anthocyanin content in the hypocotyls of soybean sprouts, thereby enhancing their nutritional and economic value. However, the mechanism by which blue light promotes anthocyanin synthesis in these hypocotyls remains to be elucidated. In this study, we identified a MYB family transcription factor, <i>GmMYB306</i>, that is highly upregulated in soybean sprout hypocotyls in response to blue light. Results showed that the expression of <i>GmMYB306</i>, an R2R3 MYB transcription factor, was positively correlated with anthocyanin synthesis in soybean. Overexpression of <i>GmMYB306</i> in hairy roots led to marked anthocyanin accumulation and significantly upregulated the expression of anthocyanin biosynthetic genes, particularly <i>GmDFR</i> and <i>GmANS</i>. Further assays demonstrated that GmMYB306 could bind to the promoters of these two genes and activate their expression. <i>GmMYB114</i> is another gene previously verified to be induced by blue light and promote anthocyanin synthesis. Here, we found that GmMYB306 could interact with GmMYB114 at the protein level to further enhance anthocyanin synthesis in soybean. Although numerous HY5 binding sites were identified in the <i>GmMYB306</i> promoter, genetic evidence indicated that HY5 does not regulate <i>GmMYB306</i> expression. Our findings provide a theoretical basis for refining the regulatory network of blue light-induced anthocyanin synthesis in soybean.</p>

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Synergistic regulation of anthocyanin synthesis in soybean sprouts under blue light by GmMYB306 and GmMYB114

  • 期刊论文

  • 出版模式：

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  • 发表日期：

    2026年7月29日

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  • Yinan Liu,
  • Wanqing Dong,
  • Jiaqi Ju,
  • Leyan Guo,
  • Nana Su

摘要

Blue light exposure significantly increases anthocyanin content in the hypocotyls of soybean sprouts, thereby enhancing their nutritional and economic value. However, the mechanism by which blue light promotes anthocyanin synthesis in these hypocotyls remains to be elucidated. In this study, we identified a MYB family transcription factor, GmMYB306, that is highly upregulated in soybean sprout hypocotyls in response to blue light. Results showed that the expression of GmMYB306, an R2R3 MYB transcription factor, was positively correlated with anthocyanin synthesis in soybean. Overexpression of GmMYB306 in hairy roots led to marked anthocyanin accumulation and significantly upregulated the expression of anthocyanin biosynthetic genes, particularly GmDFR and GmANS. Further assays demonstrated that GmMYB306 could bind to the promoters of these two genes and activate their expression. GmMYB114 is another gene previously verified to be induced by blue light and promote anthocyanin synthesis. Here, we found that GmMYB306 could interact with GmMYB114 at the protein level to further enhance anthocyanin synthesis in soybean. Although numerous HY5 binding sites were identified in the GmMYB306 promoter, genetic evidence indicated that HY5 does not regulate GmMYB306 expression. Our findings provide a theoretical basis for refining the regulatory network of blue light-induced anthocyanin synthesis in soybean.