Scholarlink

<p>Arteriovenous fistula (AVF) nonmaturation remains a significant clinical challenge in hemodialysis patients. Pre-existing structural alterations of the venous wall, including fibrosis and extracellular matrix remodeling, may impair adaptive outward remodeling of the outflow vein after arterialization. Nonlinear optical microscopy techniques such as second-harmonic generation (SHG) enable collagen-sensitive visualization of tissue architecture and may provide additional structural information beyond conventional histology. This single-center observational study included two cohorts of patients undergoing vascular access surgery. Cohort A included patients undergoing AVF creation, with venous samples obtained before anastomosis to assess pre-existing structural features in relation to maturation outcome. Cohort B included patients undergoing revision or ligation of failed AVFs, providing specimens representing advanced remodeling. All samples were evaluated using hematoxylin and eosin staining and SHG microscopy. Fibrosis and SHG-derived parameters were assessed using semiquantitative scoring systems. A total of 76 patients were included. Histological fibrosis scores did not differ between matured and failed AVFs, whereas SHG microscopy revealed significant differences. Failed AVFs demonstrated higher collagen brightness, increased fibrillar disorganization, and reduced layer preservation compared with matured AVFs. These findings suggest that SHG-derived parameters may provide additional information on venous wall remodeling not detected by conventional histology.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Pre-existing venous structural remodeling and arteriovenous fistula maturation assessed by conventional histology and nonlinear optical microscopy

  • 期刊论文

  • 出版模式：

    开放获取

  • 发表日期：

    2026年7月25日

查看全文
  • Viltė Gabrielė Samsonė,
  • Viktoras Mažeika,
  • Lukas Kontenis,
  • Jokūbas Pimpė,
  • Mykolas Maciulis,
  • Danielius Samsonas,
  • Laurynas Rimševičius,
  • Birutė Vaišnytė,
  • Simonas Jonas Norvydas,
  • Edvardas Žurauskas,
  • Virginijus Barzda,
  • Petras Purlys,
  • Marius Miglinas

摘要

Arteriovenous fistula (AVF) nonmaturation remains a significant clinical challenge in hemodialysis patients. Pre-existing structural alterations of the venous wall, including fibrosis and extracellular matrix remodeling, may impair adaptive outward remodeling of the outflow vein after arterialization. Nonlinear optical microscopy techniques such as second-harmonic generation (SHG) enable collagen-sensitive visualization of tissue architecture and may provide additional structural information beyond conventional histology. This single-center observational study included two cohorts of patients undergoing vascular access surgery. Cohort A included patients undergoing AVF creation, with venous samples obtained before anastomosis to assess pre-existing structural features in relation to maturation outcome. Cohort B included patients undergoing revision or ligation of failed AVFs, providing specimens representing advanced remodeling. All samples were evaluated using hematoxylin and eosin staining and SHG microscopy. Fibrosis and SHG-derived parameters were assessed using semiquantitative scoring systems. A total of 76 patients were included. Histological fibrosis scores did not differ between matured and failed AVFs, whereas SHG microscopy revealed significant differences. Failed AVFs demonstrated higher collagen brightness, increased fibrillar disorganization, and reduced layer preservation compared with matured AVFs. These findings suggest that SHG-derived parameters may provide additional information on venous wall remodeling not detected by conventional histology.