Scholarlink

<p>Previously, we reported the generation of founder F0 cattle and F1 offspring harboring a targeted mutation in the prion protein gene (<i>PRNP</i>) using an all-in-one vector system. This study aimed to assess the long-term viability and physiological normality of F0 through comprehensive monitoring, systemic histological examinations, molecular analyses, and germline transmission. F0 showed no behavioral abnormalities over a long-term observation of 4&#xa0;years and 9&#xa0;months and no histological defects. All F0 tissues possessed the <i>PRNP&#xa0;</i>targeted mutation, and protein analysis confirmed the absence of <i>PRNP</i> expression in the obex region. Furthermore, germline transmission (F2) of F1 frozen thawed sperm and wildtype oocytes was confirmed through in vitro fertilized blastocysts. <i>PRNP</i> mutations were successfully transmitted to F2 generation blastocysts, as evidenced by germline transmission analysis. Overall, to our knowledge, these data present the longest monitoring of <i>PRNP</i> mutated cattle (F0), including comprehensive organ analyses and germline transmission (F2) of <i>PRNP</i> mutated cattle (F1), thereby establishing the first stable <i>PRNP</i> mutated cattle line worldwide. This cattle line can serve as an invaluable biological resource for understanding bovine prion pathobiology and food security related to bovine spongiform encephalopathy.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Long-term viability and stable germline transmission of prion-free cattle over multiple generations

  • 期刊论文

  • 出版模式：

    开放获取

  • 发表日期：

    2026年7月27日

查看全文
  • Young-Chai Kim,
  • Dong-Hyeok Kwon,
  • Gyeong-Min Gim,
  • Lauren Wickersham,
  • Jeemin Ye,
  • Byeongho Moon,
  • Kyeong-Hyeon Eom,
  • Soo-Young Yum,
  • Yu-Ran Lee,
  • Hyun-Joo Sohn,
  • Hae-Eun Kang,
  • Hyunkyoung Lee,
  • Jongho Kim,
  • Bok-Kyung Ku,
  • DoYoon Kim,
  • Daejin Jung,
  • Jinyeon Park,
  • Seongeun Heo,
  • Goo Jang

摘要

Previously, we reported the generation of founder F0 cattle and F1 offspring harboring a targeted mutation in the prion protein gene (PRNP) using an all-in-one vector system. This study aimed to assess the long-term viability and physiological normality of F0 through comprehensive monitoring, systemic histological examinations, molecular analyses, and germline transmission. F0 showed no behavioral abnormalities over a long-term observation of 4 years and 9 months and no histological defects. All F0 tissues possessed the PRNP targeted mutation, and protein analysis confirmed the absence of PRNP expression in the obex region. Furthermore, germline transmission (F2) of F1 frozen thawed sperm and wildtype oocytes was confirmed through in vitro fertilized blastocysts. PRNP mutations were successfully transmitted to F2 generation blastocysts, as evidenced by germline transmission analysis. Overall, to our knowledge, these data present the longest monitoring of PRNP mutated cattle (F0), including comprehensive organ analyses and germline transmission (F2) of PRNP mutated cattle (F1), thereby establishing the first stable PRNP mutated cattle line worldwide. This cattle line can serve as an invaluable biological resource for understanding bovine prion pathobiology and food security related to bovine spongiform encephalopathy.