Scholarlink

<p>While conventional nanocarriers like liposomes are effective for small-molecule delivery, their fabrication often involves complex, multi-step processes. This work provides a proof-of-concept demonstrating a bacterial lipoprotein as a viable, genetically encoded, and self-assembling nanocarrier. We show specifically that the detergent-solubilized lipoprotein LipoMetQ from <i>Neisseria meningitidis</i> spontaneously forms micelle-like nanoparticles (termed LipoLoad), which entrap the small molecule Rose Bengal (RB) using a simple procedure of mixing and centrifugal ultrafiltration. The resulting LipoLoad: RB formulation was analyzed by dynamic light scattering and negative-stain TEM. Entrapment was found to decrease RB aggregation and enable a sustained release profile in vitro relative to the free drug. Furthermore, MTT assays performed on a subset of cancer cell lines revealed that LipoLoad: RB increased the intrinsic cytotoxic activity of RB. These results establish LipoLoad as a novel, biologically encoded nanocarrier. The facile production method, which does not require specialized equipment, and the formulation’s stability underscore the broad potential of bacterial lipoproteins as a modular platform for nanotechnology.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

LipoLoad:RB: a nanocarrier comprising bacterial lipoprotein LipoMetQ and Rose Bengal

  • 期刊论文

  • 出版模式：

    开放获取

  • 发表日期：

    2026年7月11日

查看全文
  • Qianqiao Liu,
  • Marc A. Arslanian,
  • Matthew A. Treviño,
  • Nirk E. Quispe Calla,
  • Francesca Starvaggi,
  • Naima G. Sharaf

摘要

While conventional nanocarriers like liposomes are effective for small-molecule delivery, their fabrication often involves complex, multi-step processes. This work provides a proof-of-concept demonstrating a bacterial lipoprotein as a viable, genetically encoded, and self-assembling nanocarrier. We show specifically that the detergent-solubilized lipoprotein LipoMetQ from Neisseria meningitidis spontaneously forms micelle-like nanoparticles (termed LipoLoad), which entrap the small molecule Rose Bengal (RB) using a simple procedure of mixing and centrifugal ultrafiltration. The resulting LipoLoad: RB formulation was analyzed by dynamic light scattering and negative-stain TEM. Entrapment was found to decrease RB aggregation and enable a sustained release profile in vitro relative to the free drug. Furthermore, MTT assays performed on a subset of cancer cell lines revealed that LipoLoad: RB increased the intrinsic cytotoxic activity of RB. These results establish LipoLoad as a novel, biologically encoded nanocarrier. The facile production method, which does not require specialized equipment, and the formulation’s stability underscore the broad potential of bacterial lipoproteins as a modular platform for nanotechnology.