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<p>Carbapenem-resistant hypervirulent <i>Klebsiella pneumoniae</i> (CR-HvKP) poses an increasing global threat. However, studies on virulence characteristics of CR-HvKP are limited. In this study, we investigated the virulence characteristics of 49 CRKP and 50 strains of carbapenem-sensitive <i>Klebsiella pneumoniae</i> (CSKP) from a Chinese tertiary hospital. In the <i>Galleria mellonella</i> infection model, 10 CRKP and nine CSKP strains were hypervirulent (mortality &gt; 50%). Biofilm assays showed that six CR-HvKP and six hypervirulent CSKP (CS-HvKP) exhibited moderate-to-strong biofilm formation. By PCR testing, 22 CRKP and 28 CSKP carried at least two hypervirulence genes (<i>rmpA, rmpA2, iucA, iroB, peg344</i>) and eight CRKP had truncated <i>rmpA2.</i> Whole-genome sequencing identified <i>bla</i><sub><i>KPC−2</i></sub> as the dominant carbapenemase (85.7%) and sporadic <i>bla</i><sub><i>NDM</i></sub> variants (two <i>bla</i><sub><i>NDM−1</i></sub> and one <i>bla</i><sub><i>NDM−4</i></sub>). The 49 CRKP and nine CS-HvKP were divided into 17 sequence types (STs). Notably, all nine CR-HvKP belonged to the ST11-K64 clone, whereas three CS-HvKP were ST60-K5. Complete genome analysis of a representative CR-HvKP (A40) revealed a rare plasmid, pKpA40plas3 (194,337&#xa0;bp), harboring<i> iucABCD, iutA, rmpA, rmpA2 </i>and <i>peg344</i>. This study reveals CR-HvKP virulence characteristics in Central-South China, highlighting the predominant ST11-K64 clone and a rare virulence plasmid, and emphasizes the need for enhanced surveillance.</p>

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Characteristics of ST11-K64 carbapenem-resistant hypervirulent Klebsiella pneumoniae in Central-South China

  • 期刊论文

  • 出版模式：

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  • 发表日期：

    2026年7月10日

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  • Qing-Ya Dou,
  • Meng-Li Tang,
  • An-Hua Wu,
  • Feng-Jun Xia,
  • Xun Huang,
  • Ming-Xiang Zou

摘要

Carbapenem-resistant hypervirulent Klebsiella pneumoniae (CR-HvKP) poses an increasing global threat. However, studies on virulence characteristics of CR-HvKP are limited. In this study, we investigated the virulence characteristics of 49 CRKP and 50 strains of carbapenem-sensitive Klebsiella pneumoniae (CSKP) from a Chinese tertiary hospital. In the Galleria mellonella infection model, 10 CRKP and nine CSKP strains were hypervirulent (mortality > 50%). Biofilm assays showed that six CR-HvKP and six hypervirulent CSKP (CS-HvKP) exhibited moderate-to-strong biofilm formation. By PCR testing, 22 CRKP and 28 CSKP carried at least two hypervirulence genes (rmpA, rmpA2, iucA, iroB, peg344) and eight CRKP had truncated rmpA2. Whole-genome sequencing identified blaKPC−2 as the dominant carbapenemase (85.7%) and sporadic blaNDM variants (two blaNDM−1 and one blaNDM−4). The 49 CRKP and nine CS-HvKP were divided into 17 sequence types (STs). Notably, all nine CR-HvKP belonged to the ST11-K64 clone, whereas three CS-HvKP were ST60-K5. Complete genome analysis of a representative CR-HvKP (A40) revealed a rare plasmid, pKpA40plas3 (194,337 bp), harboring iucABCD, iutA, rmpA, rmpA2 and peg344. This study reveals CR-HvKP virulence characteristics in Central-South China, highlighting the predominant ST11-K64 clone and a rare virulence plasmid, and emphasizes the need for enhanced surveillance.