Scholarlink

<p>Accurate assessment of the resection margin is of key importance for patient outcome after tumor surgery. Intraoperative assessment is preferable but present methods like fresh-frozen sectioning risk sampling errors and provide only 2D information. Classical 2D histology has better quality but is too slow for intraoperative feedback. In the present proof-of-concept study we show that laboratory x-ray histology based on propagation-based phase-contrast microtomography has potential for 3D intraoperative resection margin assessment. Our system includes a liquid–metal-jet high-brightness microfocus source, precision stages and a high-resolution detector, providing fast, high-contrast, near-cellular-resolution 3D imaging. The tissue is prepared by rapid acetone fixation before the 3D x-ray imaging. We demonstrate the method on several tumor types in liver and pancreas, as well as on different sarcomas. After the x-ray histology, classical histology was performed on the same samples for comparison. The two methods compare excellently on the liver and pancreas samples and reasonably on the morphologically more difficult sarcomas. The imaging currently takes 4–5&#xa0;h with our prototype instrument, and we outline how to go below 1&#xa0;h for the full procedure. We conclude that 3D x-ray histology can be developed to provide intraoperative resection margin assessment.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

X-ray histology on rapidly fixed fresh tumor tissue samples for fast resection margin assessment

  • 期刊论文

  • 出版模式：

    开放获取

  • 发表日期：

    2026年7月11日

查看全文
  • Jenny Romell,
  • Carlos Fernandez Moro,
  • Bertha Brodin,
  • Laszlo Szekely,
  • Edvin Porovic,
  • Panagiotis Tsagkozis,
  • Ernesto Sparrelid,
  • Mikael Björnstedt,
  • Hans M. Hertz

摘要

Accurate assessment of the resection margin is of key importance for patient outcome after tumor surgery. Intraoperative assessment is preferable but present methods like fresh-frozen sectioning risk sampling errors and provide only 2D information. Classical 2D histology has better quality but is too slow for intraoperative feedback. In the present proof-of-concept study we show that laboratory x-ray histology based on propagation-based phase-contrast microtomography has potential for 3D intraoperative resection margin assessment. Our system includes a liquid–metal-jet high-brightness microfocus source, precision stages and a high-resolution detector, providing fast, high-contrast, near-cellular-resolution 3D imaging. The tissue is prepared by rapid acetone fixation before the 3D x-ray imaging. We demonstrate the method on several tumor types in liver and pancreas, as well as on different sarcomas. After the x-ray histology, classical histology was performed on the same samples for comparison. The two methods compare excellently on the liver and pancreas samples and reasonably on the morphologically more difficult sarcomas. The imaging currently takes 4–5 h with our prototype instrument, and we outline how to go below 1 h for the full procedure. We conclude that 3D x-ray histology can be developed to provide intraoperative resection margin assessment.