<p>As a critical area for the ecological security barrier in the middle and lower reaches of the Yangtze River, the Jianghuai Watershed Area holds significant importance in coordinating ecological conservation and economic development through the assessment of its water ecosystem service value (ESV). This study focuses on Chuzhou, a typical city within the Jianghuai Watershed Area, to explore the spatiotemporal evolution of water ESV and analyze the spatiotemporal change of the Consistency between water Ecosystem services and Economy (CEE) from 2000 to 2022 based on remote sensing. Firstly, the water was extracted through normalized snow water index (NSWI) and Otsu threshold method (OTSU). Subsequently, the water ESV dynamic evaluation model was constructed. Using this model, the water ESV in Chuzhou was quantified, and its spatiotemporal distribution characteristics were analyzed. Finally, by calculating the coefficient of variation (CV) between ESV and GDP, the deviation between water ESV and GDP in Chuzhou was obtained. At the same time, the coordination between water ESV and economic development of Chuzhou was obtained by calculating CEE, so as to evaluate the Spatiotemporal coordination and regional differences between them. This study has found that: (1) The water ESV in Chuzhou showed a phased change of “fluctuating decline and subsequent recovery” from 2000 to 2022. (2) In terms of spatial distribution, the water ESV exhibits a gradient pattern of “high ESV in the northwest and low ESV in the southeast of the Chuzhou”. (3) In terms of ecological and economic coordination, the northwest of the Chuzhou maintain a healthy CEE. However, the southeast of Chuzhou has exacerbated the imbalance in CEE due to the water space occupied by construction land. This study could provide a decision-making basis for formulating ecological priority development strategies in the Jianghuai Watershed Area.</p>