<p>Acorns of <i>Quercus robur</i> L. are abundant in Finland but remain underutilized, and their chemical composition in high-latitude regions is not well documented. In this study, Finnish acorns were analyzed using gas chromatography–mass spectrometry (GC–MS) and liquid chromatography–high resolution mass spectrometry (LC–HRMS) to provide a qualitative overview of their nonpolar and polar compounds. We examined fresh whole acorns as well as dried acorn nuts and dried pericarp fractions. GC–MS analysis indicated that fresh whole acorns were characterized by signals corresponding to phytosterols and tocopherol-related compounds, whereas dried acorn nuts presented increased relative abundances of long-chain n-alkanes. Similarly, dried pericarp analysis showed sterol-related signals and long-chain aldehydes. LC–MS analysis suggested that polar fractions were characterized by ellagitannins, phenolic acids, flavonoids, and organic acids. Although the study is qualitative, it provides the first combined GC–MS and LC–MS fingerprint of the chemical profiles of Finnish <i>Quercus robur</i> L. acorns. Hence, our results provide a foundation for future quantitative studies and application-oriented research on acorn valorization.</p>

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Qualitative gas chromatography–mass spectrometry and liquid chromatography–mass spectrometry fingerprinting of tissue-specific phytochemicals in finnish Quercus robur L. acorns

  • Inga-Liina Järvinen,
  • Rahim Ullah,
  • Kari Hartonen,
  • Susanne K. Wiedmer

摘要

Acorns of Quercus robur L. are abundant in Finland but remain underutilized, and their chemical composition in high-latitude regions is not well documented. In this study, Finnish acorns were analyzed using gas chromatography–mass spectrometry (GC–MS) and liquid chromatography–high resolution mass spectrometry (LC–HRMS) to provide a qualitative overview of their nonpolar and polar compounds. We examined fresh whole acorns as well as dried acorn nuts and dried pericarp fractions. GC–MS analysis indicated that fresh whole acorns were characterized by signals corresponding to phytosterols and tocopherol-related compounds, whereas dried acorn nuts presented increased relative abundances of long-chain n-alkanes. Similarly, dried pericarp analysis showed sterol-related signals and long-chain aldehydes. LC–MS analysis suggested that polar fractions were characterized by ellagitannins, phenolic acids, flavonoids, and organic acids. Although the study is qualitative, it provides the first combined GC–MS and LC–MS fingerprint of the chemical profiles of Finnish Quercus robur L. acorns. Hence, our results provide a foundation for future quantitative studies and application-oriented research on acorn valorization.