Scholarlink

<p>COVID-19 pandemic represented an opportunity to study the relationship between viral infections and vascular damage, and how vaccination and new variants can profoundly change the clinical presentation of the disease. We studied hospitalized COVID-19 patients, mostly vaccinated and infected with SARS-CoV-2 Omicron variant, to characterize their endothelial involvement and compare it with the Alpha variant infection. Circulating endothelial cells and their ACE2 surface expression were quantified in fresh blood samples from Omicron-infected patients and controls. In parallel, proteomic profiling was performed on plasma from controls subjects and patients infected either with Omicron or Alpha variants. Finally, we evaluated endothelial toxicity and NF-κB activation in HUVECs exposed to control or patient serum. Our results shown that Omicron infection is associated with an inflammatory response leading to endothelial injury in vivo. Proteomic analysis revealed that both variants alter key vascular homeostasis proteins (IL-6, TRAIL-R2); however, the Alpha strain induces greater cytokine release and activates distinct pathways such as PARP1, Renin-Angiotensin System. Consistently, serum from Omicron-infected patients induced lower endothelial toxicity and reduced NF-κB activation compared to Alpha. Overall, our results suggests that the SARS-CoV-2 Alpha variant exerts more pronounced deleterious vascular effects, likely trough a sustaining “vasculoinflammatory” loop.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Less severe endothelial injury and vascular pathway activation in SARS-CoV-2 Omicron variant compared to Alpha

  • 期刊论文

  • 出版模式：

    开放获取

  • 发表日期：

    2026年7月3日

查看全文
  • Margherita Tiezzi,
  • Francesca Fortini,
  • Valerie Vandenbempt,
  • Javier Negueruela,
  • Desli Shahini,
  • Charbel Haddad,
  • Véronique Corbière,
  • Tatiana Takiishi,
  • Melina Houinsou Hans,
  • Patrizia Mancuso,
  • Francesco Bertolini,
  • Nicolas Baeyens,
  • Sumeet Pal Singh,
  • Esteban N. Gurzov,
  • Philippe van de Borne,
  • Alessandra K. Cardozo

摘要

COVID-19 pandemic represented an opportunity to study the relationship between viral infections and vascular damage, and how vaccination and new variants can profoundly change the clinical presentation of the disease. We studied hospitalized COVID-19 patients, mostly vaccinated and infected with SARS-CoV-2 Omicron variant, to characterize their endothelial involvement and compare it with the Alpha variant infection. Circulating endothelial cells and their ACE2 surface expression were quantified in fresh blood samples from Omicron-infected patients and controls. In parallel, proteomic profiling was performed on plasma from controls subjects and patients infected either with Omicron or Alpha variants. Finally, we evaluated endothelial toxicity and NF-κB activation in HUVECs exposed to control or patient serum. Our results shown that Omicron infection is associated with an inflammatory response leading to endothelial injury in vivo. Proteomic analysis revealed that both variants alter key vascular homeostasis proteins (IL-6, TRAIL-R2); however, the Alpha strain induces greater cytokine release and activates distinct pathways such as PARP1, Renin-Angiotensin System. Consistently, serum from Omicron-infected patients induced lower endothelial toxicity and reduced NF-κB activation compared to Alpha. Overall, our results suggests that the SARS-CoV-2 Alpha variant exerts more pronounced deleterious vascular effects, likely trough a sustaining “vasculoinflammatory” loop.