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<p>The current study aimed to produce pectinase enzyme from locally isolated fungus and to improve its industrial applicatbility as an eco-friendly alternative to chemical agents traditionally used in textile industries. The most active fungal isolate was genetically identified as <i>Aspergillus foetidus</i> (NR_163668.1). Submerged fermentation allowed maximal pectinase production yield of 138 U/mg at pH 4.5 and 37&#xa0;°C after 5 days of incubation. Among six agro-industrial substrates evaluated; 4% wheat bran, supplemented with 1% yeast extract and 0.2% EDTA resulted in maximal enzyme production. The produced pectinase showed higher stability at acidic conditions after 15 and 30&#xa0;min. Thermal kinetic parameters, including half-life (t₁/₂), enthalpy change (ΔH), Gibbs free energy (ΔG), and entropy change (ΔS), were determined, confirming high thermal stability. These properties support its potential biocatalytic application in textile industries. To confirm the feasibility of the produced pectinase enzyme in textile industry, optimized conditions were applied to treat soured polyester/cotton (PET/C) blend fabric. The reaction was performed at 50&#xa0;°C and pH of 5.0 for 40&#xa0;min. These conditions led to the removal of the sizing agent (starch) with a total weight loss of PET/C fabric of approximately 4%.</p>

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Thermodynamic studies and optimization of pectinase produced by soil-isolated Aspergillus foetidus and its application in the textile industry

  • 期刊论文

  • 出版模式：

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  • 发表日期：

    2026年6月24日

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  • Sara H. Mansour,
  • Eman F. Ahmed,
  • Naser G. Al-Balakocy,
  • Elsayed A. Elsayed

摘要

The current study aimed to produce pectinase enzyme from locally isolated fungus and to improve its industrial applicatbility as an eco-friendly alternative to chemical agents traditionally used in textile industries. The most active fungal isolate was genetically identified as Aspergillus foetidus (NR_163668.1). Submerged fermentation allowed maximal pectinase production yield of 138 U/mg at pH 4.5 and 37 °C after 5 days of incubation. Among six agro-industrial substrates evaluated; 4% wheat bran, supplemented with 1% yeast extract and 0.2% EDTA resulted in maximal enzyme production. The produced pectinase showed higher stability at acidic conditions after 15 and 30 min. Thermal kinetic parameters, including half-life (t₁/₂), enthalpy change (ΔH), Gibbs free energy (ΔG), and entropy change (ΔS), were determined, confirming high thermal stability. These properties support its potential biocatalytic application in textile industries. To confirm the feasibility of the produced pectinase enzyme in textile industry, optimized conditions were applied to treat soured polyester/cotton (PET/C) blend fabric. The reaction was performed at 50 °C and pH of 5.0 for 40 min. These conditions led to the removal of the sizing agent (starch) with a total weight loss of PET/C fabric of approximately 4%.