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<p>This study aimed to evaluate the effects of non-surgical periodontal therapy (SRP) and SRP with an amino acid-buffered hypochlorite solution (NaOCl/AA + SRP) on subgingival plaque bacteria. Quantitative changes in <i>Porphyromonas gingivalis</i>, <i>Fusobacterium nucleatum</i>, <i>Campylobacter rectus</i> and Pan Bacteria 3 assay were determined by real-time qPCR from GCF obtained from deep periodontal pocket in twelve weeks observation period. Both treatment strategies led to significant clinical improvements over time. During the first week, we observed a significant twofold decrease in the levels of <i>P. gingivalis</i> and <i>C. rectus</i> following SRP treatment. Similarly, at 12&#xa0;weeks, the between-group difference in log₂ <i>P. gingivalis</i> DNA levels (SRP vs NaOCl/AA + SRP) was −0.79 (95% CI −1.35 to −0.23; <i>p</i> = 0.006), indicating a significantly greater reduction following SRP alone. The adjunctive application of NaOCl/AA gel to SRP also resulted in reduced levels of <i>P. gingivalis, C. rectus</i>, or <i>F. nucleatum</i>; however, not as pronounced. Additionally neither treatment strategy significantly affected the total amount of bacterial DNA. In patients with low PD, a significant reduction in <i>P. gingivalis</i> expression was observed during the first week of therapy across both study groups. Conversely, in patients with high PD, a significant reduction in bacterial DNA expression was evident only in the SRP group. In conclusion, a single 30&#xa0;s NaOCl/AA gel application did not demonstrate additional benefit over SRP alone under the applied protocol.</p>

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Real-time qPCR evaluation of amino acids buffered sodium hypochlorite gel as an adjunct to non-surgical periodontal treatment: a randomized clinical trial

  • 期刊论文

  • 出版模式：

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  • 发表日期：

    2026年5月25日

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  • Ewa Dolińska,
  • Aleksandra Starosz,
  • Kamil Grubczak,
  • Katarzyna Golińska,
  • Małgorzata Pietruska

摘要

This study aimed to evaluate the effects of non-surgical periodontal therapy (SRP) and SRP with an amino acid-buffered hypochlorite solution (NaOCl/AA + SRP) on subgingival plaque bacteria. Quantitative changes in Porphyromonas gingivalis, Fusobacterium nucleatum, Campylobacter rectus and Pan Bacteria 3 assay were determined by real-time qPCR from GCF obtained from deep periodontal pocket in twelve weeks observation period. Both treatment strategies led to significant clinical improvements over time. During the first week, we observed a significant twofold decrease in the levels of P. gingivalis and C. rectus following SRP treatment. Similarly, at 12 weeks, the between-group difference in log₂ P. gingivalis DNA levels (SRP vs NaOCl/AA + SRP) was −0.79 (95% CI −1.35 to −0.23; p = 0.006), indicating a significantly greater reduction following SRP alone. The adjunctive application of NaOCl/AA gel to SRP also resulted in reduced levels of P. gingivalis, C. rectus, or F. nucleatum; however, not as pronounced. Additionally neither treatment strategy significantly affected the total amount of bacterial DNA. In patients with low PD, a significant reduction in P. gingivalis expression was observed during the first week of therapy across both study groups. Conversely, in patients with high PD, a significant reduction in bacterial DNA expression was evident only in the SRP group. In conclusion, a single 30 s NaOCl/AA gel application did not demonstrate additional benefit over SRP alone under the applied protocol.