Scholarlink

<p>Cercospora leaf spot, caused by <i>Cercospora beticola</i>, is the most destructive foliar disease of sugar beet. Management has relied heavily on fungicides, but widespread fungicide resistance emphasized the need for resistant varieties. To assess how varietal resistance shapes <i>C. beticola</i> populations, we conducted a 3-year field experiment at four locations in Germany using sugar beet varieties ranging from susceptible to highly resistant. Infected leaves were collected separately per variety and location to serve as inoculum for the following year, maintaining variety- and location-specific pathogen populations under controlled selective conditions. A total of 900 isolates were subjected to whole-genome sequencing and population genetic analyses. Isolates collected in 2022 showed extensive admixture among locations. Equal mating-type ratios were observed in some populations but skewed in others, suggesting that sexual reproduction may occur locally. Location-specific re-inoculation preserved population continuity but did not produce detectable local adaptation. By contrast, the population was affected to some extent by variety-specific re-inoculation. Populations from one highly resistant variety formed two divergent lineages with reduced diversity and altered allele frequency spectra, consistent with host-driven selection. Genome scans identified 26 candidate genes, including two putative effectors, associated with adaptation to the highly resistant variety. These findings demonstrate rapid <i>C. beticola</i> adaptation to resistant varieties, highlighting the role of host-driven selection in resistance management.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Experimental evolution reveals genomic signatures of variety-specific selection of Cercospora beticola in Germany

  • 期刊论文

  • 出版模式：

    开放获取

  • 发表日期：

    2026年5月21日

查看全文
  • Yixuan Yang,
  • Nathan A. Wyatt,
  • Ana L. Martinez,
  • Melvin D. Bolton,
  • Britt-Louise Lennefors,
  • Harald Keunecke,
  • Heinrich Reineke,
  • Maria Köhler,
  • Mark Varrelmann,
  • Sebastian Liebe

摘要

Cercospora leaf spot, caused by Cercospora beticola, is the most destructive foliar disease of sugar beet. Management has relied heavily on fungicides, but widespread fungicide resistance emphasized the need for resistant varieties. To assess how varietal resistance shapes C. beticola populations, we conducted a 3-year field experiment at four locations in Germany using sugar beet varieties ranging from susceptible to highly resistant. Infected leaves were collected separately per variety and location to serve as inoculum for the following year, maintaining variety- and location-specific pathogen populations under controlled selective conditions. A total of 900 isolates were subjected to whole-genome sequencing and population genetic analyses. Isolates collected in 2022 showed extensive admixture among locations. Equal mating-type ratios were observed in some populations but skewed in others, suggesting that sexual reproduction may occur locally. Location-specific re-inoculation preserved population continuity but did not produce detectable local adaptation. By contrast, the population was affected to some extent by variety-specific re-inoculation. Populations from one highly resistant variety formed two divergent lineages with reduced diversity and altered allele frequency spectra, consistent with host-driven selection. Genome scans identified 26 candidate genes, including two putative effectors, associated with adaptation to the highly resistant variety. These findings demonstrate rapid C. beticola adaptation to resistant varieties, highlighting the role of host-driven selection in resistance management.