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<p>The aim of this study was to determine the effect of pulsed electric field (PEF) application and soaking (coating) of rapeseeds in metal nanoparticles (AgNP or CuNP) on germination capacity, the stem and root length, the contents of chlorophyll <i>a</i>, chlorophyll <i>b</i> and carotenoids, colour profile and antioxidant properties: antioxidant activity (FRAP), polyphenols, total phenolic compounds (TPC), antioxidant capacity (ABTS) and total antioxidative activity (DPPH) of rapeseed sprouts. It was found that higher PEF energy (5.5&#xa0;kJ/kg) and soaking seeds in nanoparticle solutions (25&#xa0;ml/l and 50&#xa0;ml/l) do not affect seed germination capacity. However, a lower PEF energy (1.0&#xa0;kJ/kg) and treatment of seeds with the experimental nanoparticle solutions, in most cases, reduced rapeseed germination capacity compared with the control samples. Following the application of a 1.0&#xa0;kJ/kg PEF, stem lengths were 18.9%-48.4% higher than those of the corresponding control samples. Increased sprout root length was observed for treatments with a PEF level of 5.5&#xa0;kJ/kg, i.e., the observed values were 3.0%-103.0% higher than those for the corresponding control samples. Copper nanoparticles applied at two experimental concentrations at an energy of 1.0&#xa0;kJ/kg adversely affected root elongation; the resulting root lengths were 4.3% and 41.0% shorter than those of the respective control samples. The contents of chlorophyll <i>a</i> (chl <i>a</i>), chlorophyll <i>b</i> (chl <i>b</i>) and total carotenoids decreased in sprouts in all experimental variants compared with the control. The chlorophyll <i>a</i> content decreased by 1.89 to 11.11%, while chlorophyll <i>b</i> content decreased by 2.27 to 13.07% compared with the control. The study confirmed an increase in the antioxidant properties of rapeseed sprouts after application of silver or copper nanoparticles with a pulsed electric field. The Antioxidant activity capacity (measured by ferric reducing antioxidant power assay - FRAP), the contents of polyphenols and total phenolic compounds (TPC) ranged from 81.89 to 198.52&#xa0;mg TE/g DM, 14.23 to 15.97&#xa0;mg GAE/g DM, and 22.50 to 27.00&#xa0;mg GAE/g DM, respectively, and showed an upward trend compared with the control samples. The results of the present study suggest that further analysis is required to understand how the methods studied affect sprout development and the increased antioxidant activity.</p>

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Effects of pulsed electric field and metal nanoparticle soaking on germination, growth, and antioxidant properties of rapeseed sprouts

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  • 发表日期：

    2026年5月25日

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  • Karolina Sokal,
  • Magdalena Kachel-Górecka,
  • Małgorzata Stryjecka,
  • Marta Krajewska

摘要

The aim of this study was to determine the effect of pulsed electric field (PEF) application and soaking (coating) of rapeseeds in metal nanoparticles (AgNP or CuNP) on germination capacity, the stem and root length, the contents of chlorophyll a, chlorophyll b and carotenoids, colour profile and antioxidant properties: antioxidant activity (FRAP), polyphenols, total phenolic compounds (TPC), antioxidant capacity (ABTS) and total antioxidative activity (DPPH) of rapeseed sprouts. It was found that higher PEF energy (5.5 kJ/kg) and soaking seeds in nanoparticle solutions (25 ml/l and 50 ml/l) do not affect seed germination capacity. However, a lower PEF energy (1.0 kJ/kg) and treatment of seeds with the experimental nanoparticle solutions, in most cases, reduced rapeseed germination capacity compared with the control samples. Following the application of a 1.0 kJ/kg PEF, stem lengths were 18.9%-48.4% higher than those of the corresponding control samples. Increased sprout root length was observed for treatments with a PEF level of 5.5 kJ/kg, i.e., the observed values were 3.0%-103.0% higher than those for the corresponding control samples. Copper nanoparticles applied at two experimental concentrations at an energy of 1.0 kJ/kg adversely affected root elongation; the resulting root lengths were 4.3% and 41.0% shorter than those of the respective control samples. The contents of chlorophyll a (chl a), chlorophyll b (chl b) and total carotenoids decreased in sprouts in all experimental variants compared with the control. The chlorophyll a content decreased by 1.89 to 11.11%, while chlorophyll b content decreased by 2.27 to 13.07% compared with the control. The study confirmed an increase in the antioxidant properties of rapeseed sprouts after application of silver or copper nanoparticles with a pulsed electric field. The Antioxidant activity capacity (measured by ferric reducing antioxidant power assay - FRAP), the contents of polyphenols and total phenolic compounds (TPC) ranged from 81.89 to 198.52 mg TE/g DM, 14.23 to 15.97 mg GAE/g DM, and 22.50 to 27.00 mg GAE/g DM, respectively, and showed an upward trend compared with the control samples. The results of the present study suggest that further analysis is required to understand how the methods studied affect sprout development and the increased antioxidant activity.