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<p>Bamboo is a vital renewable resource in tropical Asia, yet its physiological responses to environmental Bamboo is an ecologically and economically important renewable resource in tropical regions, yet its physiological response to light-induced stress remains poorly characterized under in vivo conditions. In this study, time-domain optical coherence tomography (TD-OCT) was employed as a non-invasive imaging technique to monitor microstructural and optical changes in <i>Bambusa spinosa</i> leaves subjected to controlled light stress. A portable TD-OCT system was used to quantify extinction coefficients and palisade layer thickness following repeated exposure to high-intensity illumination over several days. These measurements were complemented by Vegetation Ratio (VR) index analysis derived from optical microscopy to assess pigment-related changes. The results reveal a systematic decrease in extinction coefficients in both epidermal and mesophyll boundary layers with increasing exposure duration, indicating progressive tissue degradation. Mean extinction coefficients declined from approximately 1.03&#xa0;mm⁻1 to 0.72&#xa0;mm⁻1 under prolonged illumination, while palisade layer thickness showed a decreasing trend consistent with stress-induced structural alteration. Variations in VR index values correlated with OCT-derived optical parameters, reflecting concurrent changes in leaf optical properties and physiological condition. The ability of TD-OCT to perform repeated, label-free measurements on intact leaves enables longitudinal monitoring of stress responses. These findings highlight the potential of TD-OCT combined with VR index analysis as a sensitive tool for early stress detection and precision monitoring in sustainable bamboo cultivation and environmental assessment.</p>

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Non-invasive assessment of bamboo leaf optical properties and microstructural changes under light stress using time-domain OCT

  • 期刊论文

  • 出版模式：

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  • 发表日期：

    2026年5月8日

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  • Jumar G. Cadondon,
  • Hiyam A. Halapit,
  • Maria Cecilia Galvez,
  • Tatsuo Shiina,
  • Edgar Vallar

摘要

Bamboo is a vital renewable resource in tropical Asia, yet its physiological responses to environmental Bamboo is an ecologically and economically important renewable resource in tropical regions, yet its physiological response to light-induced stress remains poorly characterized under in vivo conditions. In this study, time-domain optical coherence tomography (TD-OCT) was employed as a non-invasive imaging technique to monitor microstructural and optical changes in Bambusa spinosa leaves subjected to controlled light stress. A portable TD-OCT system was used to quantify extinction coefficients and palisade layer thickness following repeated exposure to high-intensity illumination over several days. These measurements were complemented by Vegetation Ratio (VR) index analysis derived from optical microscopy to assess pigment-related changes. The results reveal a systematic decrease in extinction coefficients in both epidermal and mesophyll boundary layers with increasing exposure duration, indicating progressive tissue degradation. Mean extinction coefficients declined from approximately 1.03 mm⁻1 to 0.72 mm⁻1 under prolonged illumination, while palisade layer thickness showed a decreasing trend consistent with stress-induced structural alteration. Variations in VR index values correlated with OCT-derived optical parameters, reflecting concurrent changes in leaf optical properties and physiological condition. The ability of TD-OCT to perform repeated, label-free measurements on intact leaves enables longitudinal monitoring of stress responses. These findings highlight the potential of TD-OCT combined with VR index analysis as a sensitive tool for early stress detection and precision monitoring in sustainable bamboo cultivation and environmental assessment.