Scholarlink

<p>The human skin microbiota is intricately linked to the microenvironmental niche of its habitat. Different skin sites may harbor distinct bacterial community structures due to their unique microenvironments. To elucidate the site-specific distribution patterns of skin bacterial communities, this study employed an integrated approach combining traditional microbial cultivation methods with 16S rRNA gene high-throughput sequencing to analyze 105 skin swab samples collected from the forehead, volar forearm, and scalp of 35 healthy individuals. A total of 675 bacterial strains encompassing 41 species and 18 genera were isolated by traditional culture-based methods. <i>Staphylococcu</i>s and <i>Cutibacterium</i> were the dominant genera in different skin sites, with <i>S. capitis</i>, <i>S. epidermidis</i>, <i>S. hominis</i>, and <i>C. acnes</i> identified as the predominant species. High-throughput sequencing analyses revealed that the forehead and forearm harbored similar microbiota profiles, while the scalp displayed a more distinct bacterial community characterized by reduced alpha diversity and elevated relative abundances of <i>Cutibacterium</i>, <i>Staphylococcus</i>, and <i>Lawsonella</i> genera. The findings from both traditional cultivation methods and high-throughput sequencing suggested that the human skin bacterial community exhibits strong site-specificity. In contrast to the significant variations observed across different skin sites, gender and age exerted minimal effects on microbiota composition. These results suggest that microenvironmental factors play a more substantial role than age- and gender-related factors in shaping the diversity and community structure of the human skin microbiota.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

A comparative analysis of skin bacteria in healthy individuals through traditional cultivation and high-throughput sequencing techniques

  • 期刊论文

  • 出版模式：

    开放获取

  • 发表日期：

    2026年4月30日

查看全文
  • Jing Feng,
  • Shaolu Zhou,
  • Jiancong Huang,
  • Xia Wen,
  • Gang Zhou,
  • Guifang Zhang,
  • Qingshan Shi,
  • Xiaobao Xie

摘要

The human skin microbiota is intricately linked to the microenvironmental niche of its habitat. Different skin sites may harbor distinct bacterial community structures due to their unique microenvironments. To elucidate the site-specific distribution patterns of skin bacterial communities, this study employed an integrated approach combining traditional microbial cultivation methods with 16S rRNA gene high-throughput sequencing to analyze 105 skin swab samples collected from the forehead, volar forearm, and scalp of 35 healthy individuals. A total of 675 bacterial strains encompassing 41 species and 18 genera were isolated by traditional culture-based methods. Staphylococcus and Cutibacterium were the dominant genera in different skin sites, with S. capitis, S. epidermidis, S. hominis, and C. acnes identified as the predominant species. High-throughput sequencing analyses revealed that the forehead and forearm harbored similar microbiota profiles, while the scalp displayed a more distinct bacterial community characterized by reduced alpha diversity and elevated relative abundances of Cutibacterium, Staphylococcus, and Lawsonella genera. The findings from both traditional cultivation methods and high-throughput sequencing suggested that the human skin bacterial community exhibits strong site-specificity. In contrast to the significant variations observed across different skin sites, gender and age exerted minimal effects on microbiota composition. These results suggest that microenvironmental factors play a more substantial role than age- and gender-related factors in shaping the diversity and community structure of the human skin microbiota.