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<p>Following the recent success of genetically modified T cells in cancer immunotherapy, an increasing number of studies are focusing on natural killer (NK) cells as an alternative to T cells. The characteristics of NK cells may help overcome the disadvantages of T cell-based therapy, such as graft-versus-host disease, cytokine release syndrome, and the high cost and long preparation time of the treatment. To accelerate the development of NK cell-based therapies, the establishment of appropriate mouse models using murine NK cells would be beneficial. Although several groups have reported the establishment of murine NK cell clones, only a few are currently available. In this study, we established a murine NK cell line, NKMS-1, which can grow in a standard cell culture medium supplemented with interleukin-2 and can be stored frozen, retaining cytotoxicity and the ability to be genetically modified. These properties make the cell line suitable for studying NK cell-based cancer immunotherapy and NK cell biology.</p>

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Establishment and characterization of NKMS-1, a novel mouse NK cell line

  • 期刊论文

  • 出版模式：

    开放获取

  • 发表日期：

    2026年5月15日

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  • Yuya Miyakura,
  • Tomoka Nagashima,
  • Shin Ito,
  • Haruna Fujimori,
  • Takayuki Imai,
  • Yukinori Asada,
  • Mai Mochizuki,
  • Keiichi Tamai,
  • Yukio Katori,
  • Jun Yasuda,
  • Kazuo Sugamura,
  • Kazunori Yamaguchi

摘要

Following the recent success of genetically modified T cells in cancer immunotherapy, an increasing number of studies are focusing on natural killer (NK) cells as an alternative to T cells. The characteristics of NK cells may help overcome the disadvantages of T cell-based therapy, such as graft-versus-host disease, cytokine release syndrome, and the high cost and long preparation time of the treatment. To accelerate the development of NK cell-based therapies, the establishment of appropriate mouse models using murine NK cells would be beneficial. Although several groups have reported the establishment of murine NK cell clones, only a few are currently available. In this study, we established a murine NK cell line, NKMS-1, which can grow in a standard cell culture medium supplemented with interleukin-2 and can be stored frozen, retaining cytotoxicity and the ability to be genetically modified. These properties make the cell line suitable for studying NK cell-based cancer immunotherapy and NK cell biology.