Scholarlink

<p>Sarcopenia, the age-related loss of skeletal muscle mass and function, represents a growing health burden with limited therapeutic options. Given the emerging roles of the gut–muscle axis and AMP-activated protein kinase (AMPK) in muscle homeostasis, we sought to identify gut-derived microbial strains that enhance muscle function via AMPK activation. We identified <i>Bifidobacterium animalis subsp. lactis</i> DS109-B11 as a potent AMPK activator. DS109-B11 microbial culture supernatant (MCS) increased AMPK phosphorylation during C2C12 myoblast differentiation, enhanced myogenic differentiation, and mitigated dexamethasone-induced myotube atrophy in vitro. In aged mice, oral administration of live DS109-B11 improved grip strength and motor performance and increased myofiber cross-sectional area, accompanied by elevated AMPK phosphorylation, upregulated mitochondrial and oxidative phosphorylation genes, and downregulated atrophy- and inflammation-related genes in skeletal muscle. In a botulinum toxin–induced neurogenic atrophy model, DS109-B11 treatment partially preserved tibialis anterior muscle mass, improved myofiber cross-sectional area, and suppressed atrophy-related gene expression. These findings identify DS109-B11 as an AMPK-activating probiotic strain that beneficially modulates skeletal muscle differentiation, enhances resilience to catabolic stress, and improves muscle function in vivo.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Probiotic Bifidobacterium animalis subsp. lactis DS109-B11 ameliorates age-related muscle weakness via AMPK activation

  • 期刊论文

  • 出版模式：

    开放获取

  • 发表日期：

    2026年4月12日

查看全文
  • Jae Won Yang,
  • Min Ju Kim,
  • Heeyeon Jeong,
  • Seongwan Kim,
  • Doo-Sang Park,
  • Yong Ryoul Yang,
  • Kwang-Pyo Lee

摘要

Sarcopenia, the age-related loss of skeletal muscle mass and function, represents a growing health burden with limited therapeutic options. Given the emerging roles of the gut–muscle axis and AMP-activated protein kinase (AMPK) in muscle homeostasis, we sought to identify gut-derived microbial strains that enhance muscle function via AMPK activation. We identified Bifidobacterium animalis subsp. lactis DS109-B11 as a potent AMPK activator. DS109-B11 microbial culture supernatant (MCS) increased AMPK phosphorylation during C2C12 myoblast differentiation, enhanced myogenic differentiation, and mitigated dexamethasone-induced myotube atrophy in vitro. In aged mice, oral administration of live DS109-B11 improved grip strength and motor performance and increased myofiber cross-sectional area, accompanied by elevated AMPK phosphorylation, upregulated mitochondrial and oxidative phosphorylation genes, and downregulated atrophy- and inflammation-related genes in skeletal muscle. In a botulinum toxin–induced neurogenic atrophy model, DS109-B11 treatment partially preserved tibialis anterior muscle mass, improved myofiber cross-sectional area, and suppressed atrophy-related gene expression. These findings identify DS109-B11 as an AMPK-activating probiotic strain that beneficially modulates skeletal muscle differentiation, enhances resilience to catabolic stress, and improves muscle function in vivo.