Scholarlink

<p>The multiple <i>Diatraea</i> (Lepidoptera: Crambidae) species comprising the stem borer complex are among the most damaging pests of sugarcane in the Americas, with <i>D. saccharalis</i>, <i>D. indigenella</i>, <i>D. busckella</i>, and <i>D. tabernella</i> considered as the most widespread species in Colombia. Entomological research in Colombia has focused on the use of egg and larval parasitoids to control populations and minimize their negative economic impacts. However, the declining effectiveness of these parasitoids on certain species has encouraged the exploration of complementary management strategies, including alternatives such as insecticidal proteins derived from <i>Bacillus thuringiensis</i> (<i>Bt</i>). This study evaluated the toxicity of the Cry1Ab protein from <i>Bt</i> against the four most widespread species of the <i>Diatraea</i> complex in Colombia using a bioassay protocol with fresh corn tissue. The mortality rate of Cry1Ab protein followed a concentration response in three of the four species tested, enabling the estimation of toxicity parameters [Lethal Concentration (LC) LC<sub>50</sub> and LC<sub>95</sub>]. Growth inhibition (<i>GI</i>) and growth index (<i>GInd - RGI</i>) were higher than 90% and lower than 0.1, respectively, for all species tested. These results demonstrate that the toxicity of Cry1Ab is similar in all four <i>Diatraea</i> species tested in this study, supporting the potential use of this <i>Bt</i> protein as an alternative management for these pest species.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Cry1Ab toxicity against four species of the Diatraea sugarcane borer complex in Colombia

  • 期刊论文

  • 出版模式：

    开放获取

  • 发表日期：

    2026年5月28日

查看全文
  • Juan Sebastián Ángel-Salazar,
  • Germán Vargas,
  • Caroline Placidi de Bortoli,
  • Juan Luis Jurat-Fuentes,
  • Claudia Echeverri-Rubiano,
  • Jershon López-Gerena,
  • Carolina Camargo

摘要

The multiple Diatraea (Lepidoptera: Crambidae) species comprising the stem borer complex are among the most damaging pests of sugarcane in the Americas, with D. saccharalis, D. indigenella, D. busckella, and D. tabernella considered as the most widespread species in Colombia. Entomological research in Colombia has focused on the use of egg and larval parasitoids to control populations and minimize their negative economic impacts. However, the declining effectiveness of these parasitoids on certain species has encouraged the exploration of complementary management strategies, including alternatives such as insecticidal proteins derived from Bacillus thuringiensis (Bt). This study evaluated the toxicity of the Cry1Ab protein from Bt against the four most widespread species of the Diatraea complex in Colombia using a bioassay protocol with fresh corn tissue. The mortality rate of Cry1Ab protein followed a concentration response in three of the four species tested, enabling the estimation of toxicity parameters [Lethal Concentration (LC) LC50 and LC95]. Growth inhibition (GI) and growth index (GInd - RGI) were higher than 90% and lower than 0.1, respectively, for all species tested. These results demonstrate that the toxicity of Cry1Ab is similar in all four Diatraea species tested in this study, supporting the potential use of this Bt protein as an alternative management for these pest species.