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<p>Outer membrane vesicles (OMVs) released by bacteria contain bacteria-specific nucleic acids and proteins. <i>Porphyromonas gingivalis</i> (<i>Pg</i>)-OMVs exert various effects on periodontal tissue cells; however, their involvement in bone metabolism remains unclear. Therefore, this study aimed to investigate the effects of <i>Pg</i>-OMVs on osteoclasts and osteoblasts in vitro and elucidate their involvement in alveolar bone resorption in vivo using experimental periodontitis model rats. Tartrate-resistant acid phosphatase staining and Western blotting revealed that <i>Pg</i>-OMVs promoted osteoclast differentiation and increased the levels of several osteoclast differentiation markers, respectively, in RAW264.7 cells. Quantitative reverse transcription-polymerase chain reaction and Western blotting revealed that <i>Pg</i>-OMVs increased the receptor activator of nuclear factor-kappa B (NF-κB) ligand (RANKL) levels and decreased osteoprotegerin (OPG) levels in MC3T3-E1 cells. <i>Pg</i>-OMVs reduced alkaline phosphatase activity and calcified nodule formation in a concentration-dependent manner. Rats were divided into four groups (healthy control, <i>Pg</i>-OMV administration, ligature-induced periodontitis, and ligature-induced periodontitis with <i>Pg</i>-OMV administration groups), and the alveolar bone resorption degree was measured via micro-computed tomography. <i>Pg</i>-OMVs administration significantly promoted alveolar bone resorption and increased the RANKL/OPG ratio in periodontal tissues. Our results suggest that <i>Pg</i>-OMVs contribute to alveolar bone resorption by promoting osteoclast differentiation, suppressing osteoblast mineralization, and increasing the RANKL/OPG ratio.</p>

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Porphyromonas gingivalis outer membrane vesicles promote alveolar bone resorption by increasing the local RANKL/OPG ratio in experimental periodontitis model rats

  • 期刊论文

  • 出版模式：

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  • 发表日期：

    2026年4月5日

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  • Daisuke Nakamura,
  • Yuji Inagaki,
  • Yuta Uemura,
  • Yuka Hiroshima,
  • Yoshimune Iwata,
  • Rie Kido,
  • Mika Bando,
  • Tomoko Sumitomo,
  • Hiromichi Yumoto

摘要

Outer membrane vesicles (OMVs) released by bacteria contain bacteria-specific nucleic acids and proteins. Porphyromonas gingivalis (Pg)-OMVs exert various effects on periodontal tissue cells; however, their involvement in bone metabolism remains unclear. Therefore, this study aimed to investigate the effects of Pg-OMVs on osteoclasts and osteoblasts in vitro and elucidate their involvement in alveolar bone resorption in vivo using experimental periodontitis model rats. Tartrate-resistant acid phosphatase staining and Western blotting revealed that Pg-OMVs promoted osteoclast differentiation and increased the levels of several osteoclast differentiation markers, respectively, in RAW264.7 cells. Quantitative reverse transcription-polymerase chain reaction and Western blotting revealed that Pg-OMVs increased the receptor activator of nuclear factor-kappa B (NF-κB) ligand (RANKL) levels and decreased osteoprotegerin (OPG) levels in MC3T3-E1 cells. Pg-OMVs reduced alkaline phosphatase activity and calcified nodule formation in a concentration-dependent manner. Rats were divided into four groups (healthy control, Pg-OMV administration, ligature-induced periodontitis, and ligature-induced periodontitis with Pg-OMV administration groups), and the alveolar bone resorption degree was measured via micro-computed tomography. Pg-OMVs administration significantly promoted alveolar bone resorption and increased the RANKL/OPG ratio in periodontal tissues. Our results suggest that Pg-OMVs contribute to alveolar bone resorption by promoting osteoclast differentiation, suppressing osteoblast mineralization, and increasing the RANKL/OPG ratio.