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<p>Precise control of the α7 nicotinic acetylcholine receptor (α7-nAChR) in specific brain cell types is crucial for understanding its role in neural circuit function and disease. Here, we introduce a set of AAV.PHP.eB-based gene delivery vectors optimized for cell-type-specific downregulation or overexpression of α7-nAChR in the adult mouse brain. Using promoters for neuronal or astrocytic targeting, we achieved efficient cell-type modulation of α7-nAChR after intra-hippocampal administration. These vectors provide a versatile platform for investigating α7-nAChR-dependent mechanisms in vivo and advancing the preclinical development of targeted interventions for neurological disorders characterized by receptor dysregulation.</p>

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AAV.PHP.eB-based strategies for precise modulation of α7 nicotinic acetylcholine receptor in neurons and astrocytes in the adult mouse brain

  • 期刊论文

  • 出版模式：

    开放获取

  • 发表日期：

    2026年4月1日

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  • Giulia Puliatti,
  • Pietro Renna,
  • Martina Battistoni,
  • Roberto Piacentini,
  • Cristian Ripoli,
  • Claudio Grassi

摘要

Precise control of the α7 nicotinic acetylcholine receptor (α7-nAChR) in specific brain cell types is crucial for understanding its role in neural circuit function and disease. Here, we introduce a set of AAV.PHP.eB-based gene delivery vectors optimized for cell-type-specific downregulation or overexpression of α7-nAChR in the adult mouse brain. Using promoters for neuronal or astrocytic targeting, we achieved efficient cell-type modulation of α7-nAChR after intra-hippocampal administration. These vectors provide a versatile platform for investigating α7-nAChR-dependent mechanisms in vivo and advancing the preclinical development of targeted interventions for neurological disorders characterized by receptor dysregulation.