Scholarlink

<p>This study provides a comprehensive analysis of the morphological-developmental characteristics of the pupal stage in <i>Drosophila</i>, a model organism for metamorphosis research. We have delineated the critical stages of pupal development, from the late third-instar larvae to adult emergence, through continuous photography and time-lapse imaging. Our findings include the identification of the head-movement phase and head-static phase as distinct transitional states preceding the pupal stage. We document pupal shell dynamics, including color transitions and spiracle development as staging markers, along with internal transformations like larval tracheal degeneration, mouthpart retraction, and the formation of the compound eyes, wings, appendages, and bristles. In conclusion, we have identified several critical periods: P12 serves as a definitive marker for the onset of segmentation during the pupal stage, P40-P50 represents an optimal period for observing the initiation of changes in eye and wing coloration, and P70 marks the beginning of the investigation into the morphological changes of the adult within the <i>Drosophila</i> pupal case. We also found that compared with <i>Canton-S (CS)</i> wild-type flies, <i>dneurexin (dnrx)</i> and <i>dneuroligins (dnlgs)</i> mutants showed some differences in development time and pupal tissue organization. These observations offer a detailed developmental map of the <i>Drosophila</i> pupal stage, which is essential for accurate experimental staging and sampling. This study’s findings not only contribute to the fundamental understanding of insect metamorphosis but also provide a valuable resource for researchers utilizing <i>Drosophila</i> as a model system to study gene expression, development, and behavior.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Dynamic morphological staging of Drosophila pupal development

  • 期刊论文

  • 出版模式：

    开放获取

  • 发表日期：

    2026年4月11日

查看全文
  • Xu Liwen,
  • Chen Mei,
  • Zhang Yumeng,
  • Pang Xuan,
  • Tang Jiaxu,
  • Zhang Chenchen,
  • Geng Junhua,
  • Xie Wei,
  • Gan Guangming

摘要

This study provides a comprehensive analysis of the morphological-developmental characteristics of the pupal stage in Drosophila, a model organism for metamorphosis research. We have delineated the critical stages of pupal development, from the late third-instar larvae to adult emergence, through continuous photography and time-lapse imaging. Our findings include the identification of the head-movement phase and head-static phase as distinct transitional states preceding the pupal stage. We document pupal shell dynamics, including color transitions and spiracle development as staging markers, along with internal transformations like larval tracheal degeneration, mouthpart retraction, and the formation of the compound eyes, wings, appendages, and bristles. In conclusion, we have identified several critical periods: P12 serves as a definitive marker for the onset of segmentation during the pupal stage, P40-P50 represents an optimal period for observing the initiation of changes in eye and wing coloration, and P70 marks the beginning of the investigation into the morphological changes of the adult within the Drosophila pupal case. We also found that compared with Canton-S (CS) wild-type flies, dneurexin (dnrx) and dneuroligins (dnlgs) mutants showed some differences in development time and pupal tissue organization. These observations offer a detailed developmental map of the Drosophila pupal stage, which is essential for accurate experimental staging and sampling. This study’s findings not only contribute to the fundamental understanding of insect metamorphosis but also provide a valuable resource for researchers utilizing Drosophila as a model system to study gene expression, development, and behavior.