Scholarlink

<p>Human muscle biopsies are often required to study or diagnose diseases. However, traditional approaches are challenging due to limited sample size, quality, or participant discomfort. Fine-gauge needle biopsies (≥ 14-gauge), present an alternative but may yield insufficient tissue for comprehensive analysis. Ultrasound guidance, coupled with vacuum-assisted, single needle-insertion multiple sampling addresses these challenges. In 19 healthy participants (mean age: 30.1 ± 10 years, 42% male), 2–3 samples were collected from a single needle insertion into the vastus lateralis (VL) and tibialis anterior (TA). Summed VL and TA sample masses averaged 148 ± 38&#xa0;mg and 166 ± 64&#xa0;mg, with dimensions of 15.83 ± 8 × 2.9 ± 0.6mm<sup>2</sup> (VL) and 15.07 ± 7 × 3.1 ± 0.9mm<sup>2</sup> (TA). VL had a mean fiber cross-sectional area of 4,347 ± 1,931µm<sup>2</sup>, with 221 ± 86 fibers quantified. Samples were of sufficient size and quality for thorough analyses from a single biopsy procedure, including mitochondrial respirometry, RT-PCR, collagen content, and biomechanical function. Fibers produced typical isometric stress values of 187&#xa0;kPa with a passive modulus of 239&#xa0;kPa (peak) and 79&#xa0;kPa (stress-relaxed). The procedure was well tolerated, with an average immediate pain rating of 1.5 ± 1 (range:0–4, scale: 0–10) and 24-hour follow-up rating of 1.7 ± 1 (range:0–4). This report describes an approach that yields high-quality muscle samples suitable for histological and biochemical analyses while minimizing discomfort.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Ultrasound-guided skeletal muscle biopsy technique permits measurement of structural, functional, cellular and biochemical properties

  • 期刊论文

  • 出版模式：

    开放获取

  • 发表日期：

    2026年3月10日

查看全文
  • Addison Barber,
  • Amber Willbanks,
  • Guadalupe Meza,
  • Jeremie L. A. Ferey,
  • Gretchen A. Meyer,
  • Sudarshan Dayanidhi,
  • W. David Arnold,
  • Richard L. Lieber,
  • Ishan Roy

摘要

Human muscle biopsies are often required to study or diagnose diseases. However, traditional approaches are challenging due to limited sample size, quality, or participant discomfort. Fine-gauge needle biopsies (≥ 14-gauge), present an alternative but may yield insufficient tissue for comprehensive analysis. Ultrasound guidance, coupled with vacuum-assisted, single needle-insertion multiple sampling addresses these challenges. In 19 healthy participants (mean age: 30.1 ± 10 years, 42% male), 2–3 samples were collected from a single needle insertion into the vastus lateralis (VL) and tibialis anterior (TA). Summed VL and TA sample masses averaged 148 ± 38 mg and 166 ± 64 mg, with dimensions of 15.83 ± 8 × 2.9 ± 0.6mm2 (VL) and 15.07 ± 7 × 3.1 ± 0.9mm2 (TA). VL had a mean fiber cross-sectional area of 4,347 ± 1,931µm2, with 221 ± 86 fibers quantified. Samples were of sufficient size and quality for thorough analyses from a single biopsy procedure, including mitochondrial respirometry, RT-PCR, collagen content, and biomechanical function. Fibers produced typical isometric stress values of 187 kPa with a passive modulus of 239 kPa (peak) and 79 kPa (stress-relaxed). The procedure was well tolerated, with an average immediate pain rating of 1.5 ± 1 (range:0–4, scale: 0–10) and 24-hour follow-up rating of 1.7 ± 1 (range:0–4). This report describes an approach that yields high-quality muscle samples suitable for histological and biochemical analyses while minimizing discomfort.