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<p>Changes in the composition and distribution of the lipids comprising the myelin sheath surrounding neuronal axons have been under-explored in neuroinflammatory diseases such as multiple sclerosis due to the complexities in the analysis of lipids in biological tissues. The application of mass spectrometry-based molecular imaging enables the study of the molecular components of demyelinating tissue. This provides a much better understanding of the complex molecular processes involved in lipid metabolism and the underlying neuroinflammatory demyelinating processes. Uncovering alterations in the lipid profiles during the demyelination processes can potentially elucidate new molecular targets for novel MS drug therapies. We have utilized mass spectrometry imaging (MSI) of in-situ sulfatide lipids in mouse CNS tissue to reveal their spatial distribution and relative abundance. We show that they are generally confined to the white matter region of the cerebellum. We provide a molecular snapshot of lipid alterations at different disease stages of experimental autoimmune encephalomyelitis (EAE), the animal model for MS. Our results suggest that alterations in sulfatide expression are greatest prior to the onset of clinical disease symptoms and are systemic through the white matter and not restricted to the focal inflammatory lesions pathognomonic of EAE and human MS. The lipid mass maps generated by MSI provide novel insights into the dynamics of lipid alterations during neuroinflammation.</p>

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MALDI-TOF mass spectrometry imaging of sulfatide lipid expression in the CNS of mice with experimental autoimmune encephalomyelitis

  • 期刊论文

  • 出版模式：

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  • 发表日期：

    2026年2月28日

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  • Krista A. Berlin,
  • Carol Chase Huizar,
  • Celeste Garza,
  • Thomas G. Forsthuber,
  • Stephan B. H. Bach

摘要

Changes in the composition and distribution of the lipids comprising the myelin sheath surrounding neuronal axons have been under-explored in neuroinflammatory diseases such as multiple sclerosis due to the complexities in the analysis of lipids in biological tissues. The application of mass spectrometry-based molecular imaging enables the study of the molecular components of demyelinating tissue. This provides a much better understanding of the complex molecular processes involved in lipid metabolism and the underlying neuroinflammatory demyelinating processes. Uncovering alterations in the lipid profiles during the demyelination processes can potentially elucidate new molecular targets for novel MS drug therapies. We have utilized mass spectrometry imaging (MSI) of in-situ sulfatide lipids in mouse CNS tissue to reveal their spatial distribution and relative abundance. We show that they are generally confined to the white matter region of the cerebellum. We provide a molecular snapshot of lipid alterations at different disease stages of experimental autoimmune encephalomyelitis (EAE), the animal model for MS. Our results suggest that alterations in sulfatide expression are greatest prior to the onset of clinical disease symptoms and are systemic through the white matter and not restricted to the focal inflammatory lesions pathognomonic of EAE and human MS. The lipid mass maps generated by MSI provide novel insights into the dynamics of lipid alterations during neuroinflammation.