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<p>Bark beetle, <i>Dendroctonus rhizophagus</i>, colonises and kills healthy sapling pine trees in the Sierra Madre Occidental, Mexico. In the autumn, its fifth-instar larvae migrate to the host’s roots (hibernaculum) to overwinter; however, little is known about temperature changes in this hibernaculum during the cold season and the physiological responses related to cold tolerance in this species. A three-year temperature record was analysed to define thermal thresholds throughout the cold season in the hibernaculum. Fifth-instar larvae were collected from the thermal thresholds and sequenced using RNA-seq to assemble a global <i>de novo</i> transcriptome. Differential expression, gene enrichment and co-expression analyses were performed to determine the main metabolic pathways and biological processes taking place in these larvae during the cold season. Three thermal thresholds were defined: late-fall, mid-winter and late-winter. In late-fall, the transcriptional response was related to motility and feeding, possibly associated with the migration of larvae to the hibernaculum; in mid-winter, it was related to the physiological adjustments involved in the cold resistance phenotype; and, in late-winter, it was related to the processes involved in pupal chamber construction and the onset of metamorphosis. Our results show that the temperature in the hibernacula and the transcriptional response of fifth-instar larvae of <i>D. rhizophagus</i> change during the cold season, where lower temperatures coincide with the cold resistance phenotype.</p>

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Temperature changes reveal different transcriptional responses in the larvae of the bark beetle Dendroctonus rhizophagus during the cold season

  • 期刊论文

  • 出版模式：

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  • 发表日期：

    2026年2月24日

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  • Moises Becerril,
  • Gerardo Zúñiga,
  • Verónica Torres-Banda,
  • María-Fernanda López,
  • Claudia Cano-Ramírez,
  • Gabriel Obregón-Molina,
  • J. Manuel Quijano-Barraza

摘要

Bark beetle, Dendroctonus rhizophagus, colonises and kills healthy sapling pine trees in the Sierra Madre Occidental, Mexico. In the autumn, its fifth-instar larvae migrate to the host’s roots (hibernaculum) to overwinter; however, little is known about temperature changes in this hibernaculum during the cold season and the physiological responses related to cold tolerance in this species. A three-year temperature record was analysed to define thermal thresholds throughout the cold season in the hibernaculum. Fifth-instar larvae were collected from the thermal thresholds and sequenced using RNA-seq to assemble a global de novo transcriptome. Differential expression, gene enrichment and co-expression analyses were performed to determine the main metabolic pathways and biological processes taking place in these larvae during the cold season. Three thermal thresholds were defined: late-fall, mid-winter and late-winter. In late-fall, the transcriptional response was related to motility and feeding, possibly associated with the migration of larvae to the hibernaculum; in mid-winter, it was related to the physiological adjustments involved in the cold resistance phenotype; and, in late-winter, it was related to the processes involved in pupal chamber construction and the onset of metamorphosis. Our results show that the temperature in the hibernacula and the transcriptional response of fifth-instar larvae of D. rhizophagus change during the cold season, where lower temperatures coincide with the cold resistance phenotype.