Scholarlink

<p><i>Mirabilis longiflora</i> L. has been used traditionally in some parts of Bangladesh for the treatment of headaches, infectious diseases, painkillers, skin disease, herpes infection, and wound healing, but its effects on multidrug-resistant (MDR) bacteria have remained unidentified. Therefore, we aimed to determine the antibacterial activity of the methanol extract of <i>M. longiflora</i> L. leaves (MEMLL) against MDR <i>Pseudomonas aeruginosa</i> and <i>Bacillus cereus</i> and to recognize possible multitargeting antibacterial phytocompounds through in silico computational approaches targeting the LasR and LpxC proteins in MDR <i>Pseudomonas aeruginosa</i> and the FosB and PlcR proteins in MDR <i>Bacillus cereus.</i> PPS, FT-IR, and GC-MS were used for profiling of the phytocompounds in MEMLL. The antimicrobial activity of MEMLL was evaluated using in vitro agar-well diffusion, MIC, and MBC assays. In silico methods were applied to identify multi-targeting agents from GC-MS-annotated phytocompounds. MEMLL showed dose-dependent antibacterial activity and exposed the presence of 33 phytochemicals in GC-MS analysis. Among these, 6-Hydroxy-4,4,7a-trimethyl-5,6,7,7a-tetrahydrobenzofuran-2(4&#xa0;H)-one (CID 14334) was identified as a potential antibacterial phytocompound as it exhibited multi-modal and strong binding affinity towards LasR, LpxC, FosB, and PlcR, favorable pharmacokinetics, drug-likeness, physicochemical, and toxicity properties. Finally, Molecular dynamics (MD) simulations demonstrated the structural stability of CID 14,334 within the active sites of LasR, LpxC, FosB, and PlcR. The results of this study offer scientific validation for the traditional use of <i>M. longiflora</i> L. in bacterial infection-related diseases. It also suggests that 6-Hydroxy-4,4,7a-trimethyl-5,6,7,7a-tetrahydrobenzofuran-2(4&#xa0;H)-one from <i>M. longiflora</i> L. might be responsible for the antibacterial activity and could act as a phytopharmacological lead for the development of LasR and LpxC inhibitors against MDR <i>Pseudomonas aeruginosa</i> and FosB and PlcR inhibitors against MDR <i>Bacillus cereus.</i></p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Uncovering the bactericidal potential of extract and multi-targeting phytochemicals from Mirabilis longiflora L. leaves against multidrug-resistant Pseudomonas aeruginosa and Bacillus cereus

  • 期刊论文

  • 出版模式：

    开放获取

  • 发表日期：

    2026年2月19日

查看全文
  • Shahina Akhter,
  • Md. Enamul Kabir Talukder,
  • Md. Tarikul Islam,
  • Md. Baha Uddin,
  • Nafis Fuad Shahir,
  • Nazia Islam Rafi,
  • Sadia Israt,
  • Rahat Alam,
  • Mohammad Abu Hena Mostofa Jamal,
  • Md. Mashiar Rahman

摘要

Mirabilis longiflora L. has been used traditionally in some parts of Bangladesh for the treatment of headaches, infectious diseases, painkillers, skin disease, herpes infection, and wound healing, but its effects on multidrug-resistant (MDR) bacteria have remained unidentified. Therefore, we aimed to determine the antibacterial activity of the methanol extract of M. longiflora L. leaves (MEMLL) against MDR Pseudomonas aeruginosa and Bacillus cereus and to recognize possible multitargeting antibacterial phytocompounds through in silico computational approaches targeting the LasR and LpxC proteins in MDR Pseudomonas aeruginosa and the FosB and PlcR proteins in MDR Bacillus cereus. PPS, FT-IR, and GC-MS were used for profiling of the phytocompounds in MEMLL. The antimicrobial activity of MEMLL was evaluated using in vitro agar-well diffusion, MIC, and MBC assays. In silico methods were applied to identify multi-targeting agents from GC-MS-annotated phytocompounds. MEMLL showed dose-dependent antibacterial activity and exposed the presence of 33 phytochemicals in GC-MS analysis. Among these, 6-Hydroxy-4,4,7a-trimethyl-5,6,7,7a-tetrahydrobenzofuran-2(4 H)-one (CID 14334) was identified as a potential antibacterial phytocompound as it exhibited multi-modal and strong binding affinity towards LasR, LpxC, FosB, and PlcR, favorable pharmacokinetics, drug-likeness, physicochemical, and toxicity properties. Finally, Molecular dynamics (MD) simulations demonstrated the structural stability of CID 14,334 within the active sites of LasR, LpxC, FosB, and PlcR. The results of this study offer scientific validation for the traditional use of M. longiflora L. in bacterial infection-related diseases. It also suggests that 6-Hydroxy-4,4,7a-trimethyl-5,6,7,7a-tetrahydrobenzofuran-2(4 H)-one from M. longiflora L. might be responsible for the antibacterial activity and could act as a phytopharmacological lead for the development of LasR and LpxC inhibitors against MDR Pseudomonas aeruginosa and FosB and PlcR inhibitors against MDR Bacillus cereus.