Scholarlink

<p>MicroRNAs (miRNAs) are key post-transcriptional regulators involved in plant development and stress responses. Although extensively studied in model species, their functional roles remain largely unexplored in non-model fruits, such as mango (<i>Mangifera indica</i> L.). In this study, we present, to our knowledge, the first genome-wide analysis of miRNAs in mango mesocarp under postharvest heat stress induced by hot water treatment (HWT), a widely used quarantine method. Using small RNA sequencing (sRNA-seq), we identified 90 miRNAs distributed across 27 families. Phylogenetic analysis revealed evolutionary trajectories shaped by whole-genome duplication. Most miRNAs were predicted to target transcription factors and regulators involved in growth and hormone signaling. Differential expression profiling across multiple time points post-HWT identified miR168, miR319, and miR482 as early heat-responsive miRNAs. Stem-loop and RT-qPCR validation revealed regulatory modules, including the miR168/<i>AGO1</i> feedback loop and the miR319/<i>TCP4-GAMYB</i> axis, involved in ROS homeostasis and thermotolerance. In addition, a transient heterologous expression assay in <i>Nicotiana benthamiana</i> demonstrated the functional repression of <i>MiTCP4</i> by miR319. Additionally, a putative interaction between miR482 and a long non-coding RNA (lncRNA) was uncovered, suggesting a potential coordination of phasiRNA generation during heat stress. These findings provide new insights into miRNA-mediated regulation in mango, highlighting both coding and non-coding networks, and lay the groundwork for future functional studies and the development of miRNA-based tools to improve thermotolerance and fruit quality.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Genome-wide analysis of conserved and novel miRNAs in mango mesocarp reveals early regulatory networks involved in postharvest heat stress response

  • 期刊论文

  • 出版模式：

    开放获取

  • 发表日期：

    2026年2月17日

查看全文
  • Mitzuko Dautt-Castro,
  • Abraham Cruz-Mendívil,
  • Libertad Ulloa-Álvarez,
  • Jorge A. Enríquez-Domínguez,
  • Miranda Ávila-Sánchez,
  • Lourdes K. Ulloa-Llanes,
  • Sergio Casas-Flores,
  • Tiago D. Serafim,
  • María A. Islas-Osuna

摘要

MicroRNAs (miRNAs) are key post-transcriptional regulators involved in plant development and stress responses. Although extensively studied in model species, their functional roles remain largely unexplored in non-model fruits, such as mango (Mangifera indica L.). In this study, we present, to our knowledge, the first genome-wide analysis of miRNAs in mango mesocarp under postharvest heat stress induced by hot water treatment (HWT), a widely used quarantine method. Using small RNA sequencing (sRNA-seq), we identified 90 miRNAs distributed across 27 families. Phylogenetic analysis revealed evolutionary trajectories shaped by whole-genome duplication. Most miRNAs were predicted to target transcription factors and regulators involved in growth and hormone signaling. Differential expression profiling across multiple time points post-HWT identified miR168, miR319, and miR482 as early heat-responsive miRNAs. Stem-loop and RT-qPCR validation revealed regulatory modules, including the miR168/AGO1 feedback loop and the miR319/TCP4-GAMYB axis, involved in ROS homeostasis and thermotolerance. In addition, a transient heterologous expression assay in Nicotiana benthamiana demonstrated the functional repression of MiTCP4 by miR319. Additionally, a putative interaction between miR482 and a long non-coding RNA (lncRNA) was uncovered, suggesting a potential coordination of phasiRNA generation during heat stress. These findings provide new insights into miRNA-mediated regulation in mango, highlighting both coding and non-coding networks, and lay the groundwork for future functional studies and the development of miRNA-based tools to improve thermotolerance and fruit quality.