Scholarlink

<p>Fungi belonging to the genus <i>Phaeoacremonium</i> are recognized as cross-kingdom pathogens, that affect both agricultural crops and human health. Here, we report the first comprehensive large-scale chemotaxonomic study based on untargeted MS-based metabolomics of 28 isolates representing 24 <i>Phaeoacremonium</i> species. The chemometric analysis was integrated with <i>in vitro</i> toxicity on human keratinocytes and cucumber (<i>Cucumis sativus</i>) cotyledons, serving as representative models for human and plant hosts, respectively. A targeted LC–MS/MS method validated the production of the naphthoquinones scytalone and isosclerone, previously associated with grapevine trunk diseases, but revealed high interspecific variability in their abundance. Chemometric analysis revealed that cultures of <i>Phaeoacremonium</i> species can be distinguished based on their chemical fingerprints. In total, 206 significant metabolic features were uncovered, spanning several chemical classes including prenol lipids, carboxylic acid derivatives, organooxygen compounds, and macrolides. Nevertheless, 36 metabolites were putatively identified. Under our experimental conditions, scytalone and isosclerone showed negligible cytotoxicity and only mild phytotoxicity. In contrast, several crude extracts gave strong toxic effects, suggesting that activity may depend on complex metabolite interactions rather than single compounds. Notably, extracts from grapevine-associated species displayed stronger phytotoxicity and cytotoxicity compared to those from olive or human sources, highlighting a more diverse or more potent array of bioactive metabolites. Together, these results expand the known chemical space of <i>Phaeoacremonium</i> and underscore the need for targeted <i>in vivo </i>studies to elucidate the ecological roles and potential health implications of their secondary metabolites.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Diversity of specialized metabolites in Phaeoacremonium species revealed by untargeted metabolomics and bioactivity assays

  • 期刊论文

  • 出版模式：

    开放获取

  • 发表日期：

    2026年2月16日

查看全文
  • Pierluigi Reveglia,
  • Maria Luisa Raimondo,
  • Carmela Paolillo,
  • Marco Masi,
  • Marcello Ziaco,
  • Lucia Lecce,
  • Thomas Conte,
  • Gaetana Ricciardi,
  • Alessio Cimmino,
  • Gaetano Corso,
  • Angelo Fontana,
  • Antonia Carlucci

摘要

Fungi belonging to the genus Phaeoacremonium are recognized as cross-kingdom pathogens, that affect both agricultural crops and human health. Here, we report the first comprehensive large-scale chemotaxonomic study based on untargeted MS-based metabolomics of 28 isolates representing 24 Phaeoacremonium species. The chemometric analysis was integrated with in vitro toxicity on human keratinocytes and cucumber (Cucumis sativus) cotyledons, serving as representative models for human and plant hosts, respectively. A targeted LC–MS/MS method validated the production of the naphthoquinones scytalone and isosclerone, previously associated with grapevine trunk diseases, but revealed high interspecific variability in their abundance. Chemometric analysis revealed that cultures of Phaeoacremonium species can be distinguished based on their chemical fingerprints. In total, 206 significant metabolic features were uncovered, spanning several chemical classes including prenol lipids, carboxylic acid derivatives, organooxygen compounds, and macrolides. Nevertheless, 36 metabolites were putatively identified. Under our experimental conditions, scytalone and isosclerone showed negligible cytotoxicity and only mild phytotoxicity. In contrast, several crude extracts gave strong toxic effects, suggesting that activity may depend on complex metabolite interactions rather than single compounds. Notably, extracts from grapevine-associated species displayed stronger phytotoxicity and cytotoxicity compared to those from olive or human sources, highlighting a more diverse or more potent array of bioactive metabolites. Together, these results expand the known chemical space of Phaeoacremonium and underscore the need for targeted in vivo studies to elucidate the ecological roles and potential health implications of their secondary metabolites.