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<p>Diabetic wounds remain a major clinical challenge due to delayed healing, persistent inflammation, and limited efficacy of existing therapies. Resveratrol possesses antioxidant, anti-inflammatory, and antibacterial properties but is restricted by poor solubility and rapid degradation. This study developed silk fibroin-coated resveratrol solid lipid nanoparticles (SF-RSLNs) to enhance resveratrol stability, provide controlled release, and improve therapeutic performance in diabetic wound healing. SF-RSLNs were fabricated using a microemulsion technique and optimized through a Box–Behnken design. The optimized nanoparticles exhibited a mean particle size of 222.1&#xa0;nm, zeta potential of − 32&#xa0;mV, entrapment efficiency of 85.7%, and drug loading of 10.2%. Characterization was performed using FTIR, SEM, and DLS analyses. In-vitro assessments included cytotoxicity (MTT), antioxidant activity (DPPH and ROS scavenging), antibacterial activity, scratch-wound healing, anti-inflammatory assays, and hemocompatibility evaluation. SF-RSLNs demonstrated sustained drug release (∼75% over 48&#xa0;h), enhanced antioxidant activity (85% DPPH scavenging at 40&#xa0;µg/mL), and significant wound closure (90% at 48&#xa0;h). Antibacterial activity was improved, with inhibition zones of 18&#xa0;mm against <i>Staphylococcus aureus</i> and 20&#xa0;mm against <i>Escherichia coli</i>. SF-RSLNs also reduced TNF-α levels by 65% and increased IL-10 levels by 50%, while exhibiting excellent hemocompatibility. These findings indicate that SF-RSLNs serve as a multifunctional nanocarrier capable of enhancing resveratrol delivery and accelerating diabetic wound repair.</p>

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Silk fibroin-coated resveratrol solid lipid nanoparticles for diabetic wound healing

  • 期刊论文

  • 出版模式：

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  • 发表日期：

    2026年2月12日

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  • Subhasri Bogadi,
  • Mohamed Rahamathulla,
  • Veera Venkata Satyanarayana Reddy Karri,
  • Gowthamarajan Kuppusamy,
  • Ali Alamri,
  • Yahya Alhamhoom,
  • Syeda Ayesha Farhana,
  • Mohammed Muqtader Ahmed,
  • Ismail Pasha

摘要

Diabetic wounds remain a major clinical challenge due to delayed healing, persistent inflammation, and limited efficacy of existing therapies. Resveratrol possesses antioxidant, anti-inflammatory, and antibacterial properties but is restricted by poor solubility and rapid degradation. This study developed silk fibroin-coated resveratrol solid lipid nanoparticles (SF-RSLNs) to enhance resveratrol stability, provide controlled release, and improve therapeutic performance in diabetic wound healing. SF-RSLNs were fabricated using a microemulsion technique and optimized through a Box–Behnken design. The optimized nanoparticles exhibited a mean particle size of 222.1 nm, zeta potential of − 32 mV, entrapment efficiency of 85.7%, and drug loading of 10.2%. Characterization was performed using FTIR, SEM, and DLS analyses. In-vitro assessments included cytotoxicity (MTT), antioxidant activity (DPPH and ROS scavenging), antibacterial activity, scratch-wound healing, anti-inflammatory assays, and hemocompatibility evaluation. SF-RSLNs demonstrated sustained drug release (∼75% over 48 h), enhanced antioxidant activity (85% DPPH scavenging at 40 µg/mL), and significant wound closure (90% at 48 h). Antibacterial activity was improved, with inhibition zones of 18 mm against Staphylococcus aureus and 20 mm against Escherichia coli. SF-RSLNs also reduced TNF-α levels by 65% and increased IL-10 levels by 50%, while exhibiting excellent hemocompatibility. These findings indicate that SF-RSLNs serve as a multifunctional nanocarrier capable of enhancing resveratrol delivery and accelerating diabetic wound repair.