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<p>This study investigated the phytochemical profile of <i>Solanum Obtusifolium Dunal</i> extract and explored its <b>biological activities</b>, such as antihyperglycemic, antiglycation, and anti-inflammatory properties. Also, it conducted computational analyses, such as molecular docking simulations. Advanced technique, including high-performance liquid chromatography with a diode-array detector, was employed to identify and quantify phytochemicals. This research evaluated the antiglycation properties of <i>Solanum Obtusifolium Dunal</i> hydro-ethanolic extract by analyzing glycation progression across different stages. The <i>in vitro</i> assessment of the antihyperglycemic effect involved the enzymes α-amylase, α-glucosidase, and pancreatic lipase. The anti-inflammatory effect was demonstrated by the inhibition of protein denaturation and the stabilization of cell membranes. Computational studies, including ADME analysis and molecular docking, were also performed. HPLC-DAD analysis revealed the significant presence of quercetin, p-coumaric acid, catechin, and gallic acid in the hydroethanolic extract from <i>Solanum Obtusifolium Dunal</i> fruit. In glycation assays, this extract exhibited targeted inhibitory effects at multiple levels. Additionally, it had a significant impact (<i>p</i> &lt; 0.001) on the activity of α-amylase, α-glucosidase and pancreatic lipase enzymes <i>in vitro</i>. This extract also exhibited a protective effect against hemolysis and protein denaturation. The findings were supported by the presence of a significant amount of flavonoids and phenolic acids, identified through HPLC-DAD analysis, along with computational studies and molecular docking, which also confirmed that the molecules of this extract were not toxic according to ADMET testing.</p>

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In vitro and in silico antiglycation antihyperglycemic and anti-inflammatory properties of chemically profiled fruit extracts of Solanum obtusifolium Dunal

  • 期刊论文

  • 出版模式：

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  • 发表日期：

    2026年2月13日

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  • Rhizlan Abdnim,
  • Mohammed Bouslamti,
  • Rafik El-Mernissi,
  • Ayoub Farihi,
  • Tarik Chelouati,
  • Ousman Brahim Mahamat,
  • Esmael M. Alyami,
  • Amir Bouallegue,
  • Mohammad Khalid,
  • Abdulrahman A. Almehizia,
  • Otmane Zouirech,
  • Mohamed Bnouham

摘要

This study investigated the phytochemical profile of Solanum Obtusifolium Dunal extract and explored its biological activities, such as antihyperglycemic, antiglycation, and anti-inflammatory properties. Also, it conducted computational analyses, such as molecular docking simulations. Advanced technique, including high-performance liquid chromatography with a diode-array detector, was employed to identify and quantify phytochemicals. This research evaluated the antiglycation properties of Solanum Obtusifolium Dunal hydro-ethanolic extract by analyzing glycation progression across different stages. The in vitro assessment of the antihyperglycemic effect involved the enzymes α-amylase, α-glucosidase, and pancreatic lipase. The anti-inflammatory effect was demonstrated by the inhibition of protein denaturation and the stabilization of cell membranes. Computational studies, including ADME analysis and molecular docking, were also performed. HPLC-DAD analysis revealed the significant presence of quercetin, p-coumaric acid, catechin, and gallic acid in the hydroethanolic extract from Solanum Obtusifolium Dunal fruit. In glycation assays, this extract exhibited targeted inhibitory effects at multiple levels. Additionally, it had a significant impact (p < 0.001) on the activity of α-amylase, α-glucosidase and pancreatic lipase enzymes in vitro. This extract also exhibited a protective effect against hemolysis and protein denaturation. The findings were supported by the presence of a significant amount of flavonoids and phenolic acids, identified through HPLC-DAD analysis, along with computational studies and molecular docking, which also confirmed that the molecules of this extract were not toxic according to ADMET testing.