Scholarlink

<p>Mare milk is rich in nutrients and bioactive compounds, suggesting that its Small extracellular vesicles may possess immunomodulatory properties. In this study, we developed an optimized ultracentrifugation method to isolate Mare Milk-Derived Small Extracellular Vesicles (MM-sEVs) from mare milk, achieving higher yield and purity compared to conventional approaches. The isolated MM-sEVs were characterized using transmission electron microscopy (TEM), nanoparticle tracking analysis (NTA), and Western blotting. Proteomic and microarray analyses revealed that MM-sEVs are enriched with immune-related proteins (e.g., RHOA) and microRNAs (e.g., miR-155). When co-cultured with RAW264.7 macrophages, MM-sEVs significantly enhanced phagocytic activity, reduced cell migration, upregulated IL-10 expression, and modulated the expression of pro-inflammatory cytokines such as IL-1β and IL-6, etc., while IFN-γ levels remained unchanged. These findings highlight the immunomodulatory potential of MM-sEVs, particularly in regulating macrophage functions, and provide novel evidence supporting their prospective applications in health-related fields.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Impact of mare milk-derived small extracellular vesicles on proliferation, phagocytosis, and migration in RAW264.7 macrophage

  • 期刊论文

  • 出版模式：

    开放获取

  • 发表日期：

    2026年2月2日

查看全文
  • Shuang Wang,
  • Qingkai Lan,
  • Siren Badama,
  • Buhe Nashun,
  • Yun Wu,
  • Yujing Bai,
  • Rihua Xu,
  • La Ta,
  • Xilinqiqige Bao,
  • Jun Fang,
  • Nagongbilige He,
  • Tserentsoo Byambaa,
  • Tsend-Ayush Damba,
  • Tserendavga Dalkh,
  • Qin Si

摘要

Mare milk is rich in nutrients and bioactive compounds, suggesting that its Small extracellular vesicles may possess immunomodulatory properties. In this study, we developed an optimized ultracentrifugation method to isolate Mare Milk-Derived Small Extracellular Vesicles (MM-sEVs) from mare milk, achieving higher yield and purity compared to conventional approaches. The isolated MM-sEVs were characterized using transmission electron microscopy (TEM), nanoparticle tracking analysis (NTA), and Western blotting. Proteomic and microarray analyses revealed that MM-sEVs are enriched with immune-related proteins (e.g., RHOA) and microRNAs (e.g., miR-155). When co-cultured with RAW264.7 macrophages, MM-sEVs significantly enhanced phagocytic activity, reduced cell migration, upregulated IL-10 expression, and modulated the expression of pro-inflammatory cytokines such as IL-1β and IL-6, etc., while IFN-γ levels remained unchanged. These findings highlight the immunomodulatory potential of MM-sEVs, particularly in regulating macrophage functions, and provide novel evidence supporting their prospective applications in health-related fields.