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<p>Semen cryopreservation results in decreased viability and fertilizing ability due to temperature variation-mediated cryodamage limiting its practical application. Though many factors play a role in the success of semen cryopreservation, ice crystal formation plays an important role in post-thaw viability because of the position and surface area to semen volume ratio of the container. The present study was to compare the post-thaw cryo-survivability of sheep semen cryopreserved in cryovials with a smaller surface area to semen volume ratio in the vertical position versus straws with a larger ratio in both horizontal and vertical positions. Significantly (<i>p</i> &lt; 0.05) higher post-thaw functional parameters and subsequent embryo production were observed in the semen cryopreserved in cryovials with LN2 vapour than cryopreserved in straws with a bio-freezer and LN2 vapour. The study concluded that higher post-thaw viability of the sperm cryopreserved in cryovials in the vertical position might be due to gravity-supported settling of the sperm at the bottom, creating a small air gap, and resulting in less ice crystal formation and subsequent embryo production in vitro. Whereas the straws in the horizontal position created a large air gap and faster ice crystal formation, and uneven distribution of temperature along the length of the straw in the vertical position leads to significant (<i>p</i> &lt; 0.05) sperm damage for subsequent embryo production.</p>

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Cryovial versus straw for sheep semen cryopreservation: a comparative study of surface area-to-volume ratio on post-thaw viability and in vitro embryo production

  • 期刊论文

  • 出版模式：

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  • 发表日期：

    2026年1月31日

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  • Anupama Karimpat,
  • Suchita Atreya,
  • Ashish Mishra,
  • Sudhir Chandra Roy,
  • Artabandhu Sahoo

摘要

Semen cryopreservation results in decreased viability and fertilizing ability due to temperature variation-mediated cryodamage limiting its practical application. Though many factors play a role in the success of semen cryopreservation, ice crystal formation plays an important role in post-thaw viability because of the position and surface area to semen volume ratio of the container. The present study was to compare the post-thaw cryo-survivability of sheep semen cryopreserved in cryovials with a smaller surface area to semen volume ratio in the vertical position versus straws with a larger ratio in both horizontal and vertical positions. Significantly (p < 0.05) higher post-thaw functional parameters and subsequent embryo production were observed in the semen cryopreserved in cryovials with LN2 vapour than cryopreserved in straws with a bio-freezer and LN2 vapour. The study concluded that higher post-thaw viability of the sperm cryopreserved in cryovials in the vertical position might be due to gravity-supported settling of the sperm at the bottom, creating a small air gap, and resulting in less ice crystal formation and subsequent embryo production in vitro. Whereas the straws in the horizontal position created a large air gap and faster ice crystal formation, and uneven distribution of temperature along the length of the straw in the vertical position leads to significant (p < 0.05) sperm damage for subsequent embryo production.