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<p>The aim of the present in vitro study was to assess the simultaneous effect of low-level laser therapy (LLLT) and leucocyte platelet-rich fibrin (L-PRF) as adjunctive therapy on gingival tissue healing. Human gingival fibroblasts (HGFs) were cultured and subjected to different concentrations of L-PRF (100%, 50%, 25%, 12.5%, and 6.25%) and 915 nm diode laser with different energy densities (2, 3, and 4 J/cm<sup>2</sup>). Cell viability, proliferation, and migration were assessed via MTT, flow cytometry, and scratch assay, respectively. The expression of connective tissue healing-related biomarkers (MMP1, ANG1, and COL1A1) was assessed via reverse transcription quantitative polymerase chain reaction (RT-qPCR). Each condition was performed in six biological replicates (n = 6). Simultaneous application of 3 J/cm<sup>2</sup> diode laser and L-PRF resulted in the highest cell viability and proliferation (<i>p</i> &lt; 0.01). The solitary application of L-PRF showed the highest cell migration (<i>p</i> &lt; 0.026). While the solitary application of L-PRF yielded the highest MMP1 expression, the simultaneous application of L-PRF and 3 J/cm<sup>2</sup> laser resulted in the highest expression of ANG1 and COL1A1 (<i>p</i> &lt; 0.01). The discrete application of 915 nm diode laser and L-PRF increased cell viability, proliferation, migration, and expression of gingival tissue healing-related biomarkers. However, within the limitations of this in-vitro study, the combined application of L-PRF with diode laser at 3 J/cm<sup>2</sup> resulted in the highest bio-stimulatory effects on HGFs.</p>

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Effect of simultaneous application of low-level diode laser and leukocyte platelet-rich fibrin on gingival tissue healing: an in vitro investigation

  • 期刊论文

  • 出版模式：

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  • 发表日期：

    2026年2月3日

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  • Hamid Nazari,
  • Mahshid Hodjat,
  • Sogol Saberi,
  • Faranak Noori,
  • Sahar Hemmatian,
  • Amir Raee

摘要

The aim of the present in vitro study was to assess the simultaneous effect of low-level laser therapy (LLLT) and leucocyte platelet-rich fibrin (L-PRF) as adjunctive therapy on gingival tissue healing. Human gingival fibroblasts (HGFs) were cultured and subjected to different concentrations of L-PRF (100%, 50%, 25%, 12.5%, and 6.25%) and 915 nm diode laser with different energy densities (2, 3, and 4 J/cm2). Cell viability, proliferation, and migration were assessed via MTT, flow cytometry, and scratch assay, respectively. The expression of connective tissue healing-related biomarkers (MMP1, ANG1, and COL1A1) was assessed via reverse transcription quantitative polymerase chain reaction (RT-qPCR). Each condition was performed in six biological replicates (n = 6). Simultaneous application of 3 J/cm2 diode laser and L-PRF resulted in the highest cell viability and proliferation (p < 0.01). The solitary application of L-PRF showed the highest cell migration (p < 0.026). While the solitary application of L-PRF yielded the highest MMP1 expression, the simultaneous application of L-PRF and 3 J/cm2 laser resulted in the highest expression of ANG1 and COL1A1 (p < 0.01). The discrete application of 915 nm diode laser and L-PRF increased cell viability, proliferation, migration, and expression of gingival tissue healing-related biomarkers. However, within the limitations of this in-vitro study, the combined application of L-PRF with diode laser at 3 J/cm2 resulted in the highest bio-stimulatory effects on HGFs.