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<p>Banana leaf diseases are a significant threat to Cavendish banana production. In the Philippines, the main disease has been diagnosed as Black sigatoka disease caused by <i>Pseudocercospora fijiensis</i> based on symptoms. However, our study showed that the main pathogen in Mindanao island, the largest banana-producing region in the Philippines, belongs to the genus <i>Nigrospora</i>, contradicting previous assumptions. We clarified the phylogenetic positions of 160 <i>Nigrospora</i> isolates based on molecular phylogenetic analyses using ITS, <i>β-tubulin</i>, and <i>tef1α</i> sequences, and compared their morphology with known species. Molecular phylogenetic and morphological analysis revealed that <i>Nigrospora</i> isolates comprised <i>N. chinensis</i>, <i>N. lacticolonia</i>, <i>N.</i> cf. <i>singularis</i>, <i>N. sphaerica</i>, <i>N. vesicularifera</i>, and a novel species, <i>N. nigrocolonia</i>. Pathogenicity tests on banana leaves confirmed that these species are pathogenic. Species other than <i>N. sphaerica</i> were for the first time reported as pathogens of banana leaf. The results of the fungicide sensitivity test using 14 fungicides, including pyrimethanil, spiroxamine, and tebuconazole, for the Sigatoka disease showed 100% inhibition of all isolates at 100&#xa0;ppm of active ingredients. However, low-sensitivity isolates were observed for the remaining 11 fungicides. Our findings indicated the need for a comprehensive review of banana leaf disease prevention strategies.</p>

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Occurrence of Nigrospora spp. as the predominant causal agents of leaf spot disease in Cavendish banana in banana plantations in Mindanao Island, Philippines

  • 期刊论文

  • 出版模式：

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  • 发表日期：

    2026年3月8日

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  • Shunsuke Nozawa,
  • Yui Harada,
  • Yoshiki Takata,
  • Keiko Uchida,
  • Mike Andre Malonzo,
  • Reynaldo Valle,
  • Sherman M. Chavez,
  • Aniway F. Penalosa,
  • Kyoko Watanabe

摘要

Banana leaf diseases are a significant threat to Cavendish banana production. In the Philippines, the main disease has been diagnosed as Black sigatoka disease caused by Pseudocercospora fijiensis based on symptoms. However, our study showed that the main pathogen in Mindanao island, the largest banana-producing region in the Philippines, belongs to the genus Nigrospora, contradicting previous assumptions. We clarified the phylogenetic positions of 160 Nigrospora isolates based on molecular phylogenetic analyses using ITS, β-tubulin, and tef1α sequences, and compared their morphology with known species. Molecular phylogenetic and morphological analysis revealed that Nigrospora isolates comprised N. chinensis, N. lacticolonia, N. cf. singularis, N. sphaerica, N. vesicularifera, and a novel species, N. nigrocolonia. Pathogenicity tests on banana leaves confirmed that these species are pathogenic. Species other than N. sphaerica were for the first time reported as pathogens of banana leaf. The results of the fungicide sensitivity test using 14 fungicides, including pyrimethanil, spiroxamine, and tebuconazole, for the Sigatoka disease showed 100% inhibition of all isolates at 100 ppm of active ingredients. However, low-sensitivity isolates were observed for the remaining 11 fungicides. Our findings indicated the need for a comprehensive review of banana leaf disease prevention strategies.